Induction of MMP-3 by hyaluronan oligosaccharides in temporomandibular joint chondrocytes
Induction of MMP-3 by hyaluronan oligosaccharides in temporomandibular joint chondrocytes
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DOI：
10.1177/154405910508401107
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发表时间：
2005-11-01
影响因子：
7.6
通讯作者：
Knudson, W
中科院分区：
文献类型：
--
作者：
Ohno, S;Ohno-Nakahara, M;Knudson, W
低分子量透明质酸（LMW - HA）在骨关节炎关节中常常增多；然而，其在软骨中的生物学功能尚未阐明。我们假设LMW - HA通过与CD44相互作用导致软骨细胞的分解代谢激活。对源自牛颞下颌关节（TMJ）的软骨外植体和软骨细胞在透明质酸寡糖（HA（寡糖））处理后进行了基质丢失和基质金属蛋白酶 - 3（MMP - 3）表达的检测。透明质酸和CD44均匀分布在TMJ髁突的纤维区和软骨区。用HA（寡糖）处理软骨外植体导致软骨基质丢失，分泌的酪蛋白水解活性增加。对TMJ软骨细胞进行HA（寡糖）处理导致MMP - 3表达增强，而在实验期间中途洗去HA（寡糖）则降低了这种诱导作用。这些结果表明HA（寡糖）激活软骨细胞，导致蛋白酶表达大幅增强，并且通过洗去HA（寡糖）可逆转这种分解代谢激活。缩写：mRNA，信使核糖核酸；hr，小时；min，分钟；DNA，脱氧核糖核酸；SDS，十二烷基硫酸钠；PAGE，聚丙烯酰胺凝胶电泳；GAPDH，甘油醛 - 3 - 磷酸脱氢酶；IgG，免疫球蛋白G；HA，透明质酸；LMW - HA，低分子量透明质酸；HMW - HA，高分子量透明质酸；HA（寡糖），透明质酸寡糖；HA6，透明质酸六糖；OA，骨关节炎；TMJ，颞下颌关节；MMP - 3，基质金属蛋白酶 - 3；RT - PCR，逆转录酶/聚合酶链反应；DMEM，达尔伯克改良伊格尔培养基；FBS，胎牛血清；DAPI，4', 6 - 二脒基 - 2 - 苯基吲哚，二盐酸盐；CP，交叉点的循环数
Low-molecular-weight hyaluronan (LMW-HA) is often increased in osteoarthritic joints; however, its biological function in cartilage has not been clarified. We hypothesize that LMW-HA causes the catabolic activation of chondrocytes through its interaction with CD44. Cartilage explants and chondrocytes, derived from bovine temporomandibular joints (TMJ), were examined for matrix loss and the expression of matrix metalloproteinase-3 (MMP-3) following treatment with hyaluronan oligosaccharides (HA(oligos)). Hyaluronan and CD44 were uniformly distributed throughout the fibrous and cartilaginous zones of the TMJ condyle. Treatment of cartilage explants with HA(oligos) resulted in cartilage matrix loss with increased secreted caseinolytic activity. HA(oligos) treatment of TMJ chondrocytes resulted in enhanced MMP-3 expression, whereas wash-out of the HA(oligos) in the middle of the experimental period reduced this induction. These results suggest that HA(oligos) activate chondrocytes, resulting in a substantial enhancement of proteinase expression and the removal of HA(oligos) by wash-out reverses this catabolic activation. Abbreviations: mRNA, messenger ribonucleic acid; hr, hour; min, minute; DNA, deoxyribonucleic acid; SDS, sodium dodecyl sulfate; PAGE, polyacrylamide gel electrophoresis; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IgG, immunoglobulin G; HA, hyaluronan; LMW-HA, low-molecular-weight hyaluronan; HMW-HA, high-molecular-weight hyaluronan; HA(oligos), hyaluronan oligosaccharides; HA6, hyaluronan hexasaccharides; OA, osteoarthritis; TMJ, temporomandibular joint; MMP-3, metalloproteinase-3; RT-PCR, reverse-transcriptase/ polymerase chain-reaction; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; DAPI. 4', 6-diamidino-2-phenylindole, dihydro-chloride CP, cycle number at the crossing point.