The effects of DNA containing CpG motif on dendritic cells
The effects of DNA containing CpG motif on dendritic cells
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DOI：
10.1046/j.1365-2567.2000.00979.x
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发表时间：
2000-03-01
期刊：
影响因子：
6.4
通讯作者：
Austyn, J
中科院分区：
文献类型：
--
作者：
Behboudi, S;Chao, D;Austyn, J
树突状细胞（DC）是特化的抗原呈递细胞。DC能够在成熟并迁移至次级淋巴组织之前在外周获取和加工抗原，在次级淋巴组织中它们呈递抗原并向T细胞传递共刺激信号。我们描述了一种含有CpG基序的免疫刺激寡核苷酸，它在体外刺激小鼠DC上调共刺激分子，诱导T细胞增殖反应并分泌白细胞介素 - 12。给小鼠施用这种寡核苷酸，而不是缺乏该基序的对照寡核苷酸，会导致DC从脾脏的边缘区和T细胞区消失，但不会从心脏或肾脏消失。相同的CpG在体外不会引起单核细胞衍生的人DC成熟，但脂多糖处理的单核细胞衍生的DC显示出增强的功能活性和上调的共刺激分子。
Dendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up-regulate co-stimulatory molecules, induce T-cell proliferative responses and secrete interleukin-12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T-cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte-derived human DC in vitro, but lipopolysaccharide-treated monocyte-derived DC showed enhanced functional activity and up-regulated co-stimulatory molecules.