Reappraisal of physiological attributes of nine strains of Dunaliella (Chlorophyceae):: Growth and pigment content across a salinity gradient
Reappraisal of physiological attributes of nine strains of Dunaliella (Chlorophyceae):: Growth and pigment content across a salinity gradient
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DOI：
10.1046/j.1529-8817.2001.037002334.x
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发表时间：
2001-04-01
影响因子：
2.9
通讯作者：
Aguilera, A
中科院分区：
文献类型：
--
作者：
Cifuentes, AS;González, MA;Aguilera, A
在用于限定杜氏盐藻亚属内的部分的少数分类学特征中，包括对盐浓度变化的生理反应，其限定了最佳生长的特定范围，以及营养细胞的胡萝卜素生成能力，其负责在次优培养条件下细胞颜色从绿色变为橙子或红色。以前的工作基于该亚属不同组的7个分类群的分子数据，表明从内转录间隔区RFLP数据推断的遗传关系与分类学中使用的形态生理学属性之间没有相关性。parva Lerche UTEX 1983和CCMP 362，D，salina Teodoresco UTEX 200，D，viridis Teodoresco CONC 002和D，peircei Nicolai et Baas Becking UTEX 2192）加入D. parva CCAP 19/9和D，pseudosalina Massyuk et拉琴科CONC 010，在广泛的NaCl浓度范围内的生长反应和色素含量进行了评估。结果表明，两株D. parva、D. tertiolecta和一株D. peircei在整个盐浓度范围内的生长反应表现出相似性。另一方面，杜氏藻（Dunaliella pan，UTEX 1983）显示出与其同种藻非常不同的生长速率模式，而与D，viridis CONC 002和D，salina UTEX 200的生长速率模式相似，就色素含量而言，没有一个菌株在测定的整个盐浓度范围内变成橙子或红色，总类胡萝卜素与叶绿素的比值始终低于或等于1.0。这些结果重申了用于区分杜氏盐藻亚属内的部分的生理属性的有效性，并显示与以前的分子数据的相关性，但强调需要将一些菌株重新定位到不同的部分。
Among the few taxonomic characters used to circumscribe sections within the subgenus Dunaliella are the physiological response to changes in salt concentration, which define a specific range for optimal growth, and the carotenogenic ability of the vegetative cells, responsible for the change in cell color from green to orange or red, under suboptimal culture conditions. Previous work based on molecular data from seven taxa of different sections of the subgenus showed no correlation between the genetic relationship inferred from the internal transcribed spacer RFLP data and the morphophysiological attributes in use in taxonomy, The present work was performed to experimentally reevaluate the physiological attributes in the same seven previous taxa (D, tertiolecta Butcher UTEX 999 and CCMP 1320, D. parva Lerche UTEX 1983 and CCMP 362, D, salina Teodoresco UTEX 200, D, viridis Teodoresco CONC 002, and D, peircei Nicolai et Baas Becking UTEX 2192) adding D. parva CCAP 19/9 and D, pseudosalina Massyuk et Radchenko CONC 010, Growth responses and pigment content in a wide range of NaCl concentrations were assessed. The results revealed that two strains of D. parva, two strains of D. tertiolecta, and one strain of D, peircei showed similarity in their growth responses to the whole range of salt concentrations. Dunaliella pan,a UTEX 1983, on the other hand, showed a growth rate pattern very different from those of their conspecifics and similar to those of D, viridis CONC 002 and D, salina UTEX 200, In relation to the pigment content, none of the strains turned orange or red in color under the whole range of salt concentrations assayed, and the total carotenoids to chlorophyll ratios were always lower or equal to 1.0. These results reaffirm the validity of the physiological attributes used to discriminate sections within the subgenus Dunaliella and show correlation with the previous molecular data, but stress the need for relocating some strains into different sections.