Association between genetic variability and titre of Grapevine Pinot gris virus with disease symptoms
Association between genetic variability and titre of Grapevine Pinot gris virus with disease symptoms
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DOI：
10.1111/ppa.12639
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发表时间：
2017-08-01
期刊：
影响因子：
2.7
通讯作者：
Angelini, E.
中科院分区：
文献类型：
--
作者：
Bertazzon, N.;Forte, V.;Angelini, E.
本工作进行，以验证一个新的葡萄疾病，其特征在于叶片斑驳和变形，和葡萄藤灰比诺病毒（GPGV），最近确定的病毒暂时与病理的遗传变异和浓度之间的可能关联。在葡萄园调查和建立实时qPCR测定之后，在从植物收集的100多个葡萄藤Glera样品中评估了GPGV分离株的表征和GPGV滴度的评价，无论它们是否显示出病毒学。结果表明，GPGV变种与症状表现之间存在重要联系，并且有症状的葡萄藤的GPGV滴度显着高于无症状的葡萄藤。此外，还发现来自有症状植物的分离物的系统发育聚类与该疾病的一些流行病学特征之间存在有趣的关系，目前的研究证实了GPGV在以葡萄藤叶片斑点和变形为特征的突发疾病中的作用。
The present work was carried out in order to verify the possible association between a new grapevine disease, characterized by leaf mottling and deformation, and the genetic variability and concentration of Grapevine Pinot gris virus (GPGV), a recently identified virus tentatively associated with the pathology. After vineyard surveys and the establishment of real-time qPCR assays, characterization of GPGV isolates and evaluation of GPGV titre were assessed in more than 100 samples of grapevine Glera, collected from plants regardless of whether or not they showed the symptomatology. Results showed that there was an important association between the GPGV variants and manifestation of the symptoms, and that grapevines with symptoms harboured significantly higher GPGV titre than symptomless vines. Moreover, an interesting relationship among the phylogenetic clustering of the isolates originating from plants with symptoms and some epidemiological characteristics of the disease was found. The current study confirmed the role of GPGV in the emergent disease characterized by grapevine leaf mottling and deformation.