Clinical potential of miRNA-221 as a novel prognostic biomarker for hepatocellular carcinoma
Clinical potential of miRNA-221 as a novel prognostic biomarker for hepatocellular carcinoma
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DOI：
10.3233/cbm-161671
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Yang, Shun-E
中科院分区：
文献类型：
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作者：
Chen, Fan;Li, Xin-Feng;Yang, Shun-E
miRNA-221是目前已知的700多种microRNA（miRNAs）之一，在多种肿瘤中表达上调，提示其可能是一种潜在的致癌miRNA。关于miRNA 221与肝细胞癌（HCC）关系的研究较少。我们采用实时定量聚合酶链反应（qPCR）方法检测肝癌及癌旁组织中miRNA-221的表达，探讨miRNA-221的异常表达与肝癌患者临床病理特征的关系。miRNA-221在肝癌组织中的表达显著高于癌旁组织（P < 0.001）。我们分析了miRNA-221表达水平与HCC患者临床病理特征的关系。结果表明，miRNA-221的表达水平与HCC患者的肿瘤分期（P = 0.012）、肿瘤结节数（P = 0.018）和微血管浸润（P = 0.010）密切相关。生存分析结果提示，miRNA-221表达上调的HCC患者生存时间较短。miRNA-221的高表达提示HCC患者预后不良，miRNA-221可作为HCC预后的重要分子标志物。
miRNA-221 is one of the over 700 kinds of currently known microRNAs (miRNAs) and is up-regulated in multiple tumors, suggesting that it may be a potential carcinogenic miRNA. Few studies have explored the relationship between miRNA221 and hepatocellular carcinoma (HCC). We performed real-time quantitative polymerase chain reaction (qPCR) to detect miRNA-221 expression in HCC and para-carcinoma tissues and to explore the relationship between abnormal expression of miRNA-221 and clinicopathological features of HCC patients. miRNA-221 expression was significantly higher in HCC tissues than in adjacent tissues (P < 0.001). We analyzed the relationship between miRNA-221 expression level and clinicopathological characteristics of HCC patients. Our results suggested that miRNA-221 expression level was closely related to tumor stage (P = 0.012), number of tumor nodes (P = 0.018), and microvascular invasion (P = 0.010) in HCC patients. The results of survival analysis suggested that HCC patients with up-regulated miRNA-221 expression had a shorter survival time. The high miRNA221 expression indicates the poor prognosis of HCC patients; thus, miRNA-221 can be regarded an important molecular marker for HCC prognosis.