Zygotic amplification of secondary piRNAs during silkworm embryogenesis
Zygotic amplification of secondary piRNAs during silkworm embryogenesis
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DOI：
10.1261/rna.2709411
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发表时间：
2011-07-01
期刊：
影响因子：
4.5
通讯作者：
Katsuma, Susumu
中科院分区：
文献类型：
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作者：
Kawaoka, Shinpei;Arai, Yuji;Katsuma, Susumu
PIWI相互作用RNA（piRNA）是23-30个核苷酸长的小RNA，其在动物性腺中充当转座因子的序列特异性沉默子。在果蝇中，遗传学和深度测序数据已经导致了一种称为乒乓循环的皮尔纳生物发生假说，其中反义初级piRNA启动扩增环以产生有义次级piRNA。然而，到目前为止，乒乓循环的过程从未在工作中受到监测。在这里，通过对来自家蚕卵巢和不同发育阶段的胚胎的piRNA进行大规模分析，我们证明了母系遗传的反义偏置piRNA在与有义转座子mRNA的合子转录一致的时间触发合子中次级有义皮尔纳产生的急性扩增。这些结果为乒乓循环提供了现场证据。
PIWI-interacting RNAs (piRNAs) are 23-30-nucleotide-long small RNAs that act as sequence-specific silencers of transposable elements in animal gonads. In flies, genetics and deep sequencing data have led to a hypothesis for piRNA biogenesis called the ping-pong cycle, where antisense primary piRNAs initiate an amplification loop to generate sense secondary piRNAs. However, to date, the process of the ping-pong cycle has never been monitored at work. Here, by large-scale profiling of piRNAs from silkworm ovary and embryos of different developmental stages, we demonstrate that maternally inherited antisense-biased piRNAs trigger acute amplification of secondary sense piRNA production in zygotes, at a time coinciding with zygotic transcription of sense transposon mRNAs. These results provide on-site evidence for the ping-pong cycle.