Live and killed vaccines against toxoplasmosis in mice.
Live and killed vaccines against toxoplasmosis in mice.
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DOI：
10.2307/3281275
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发表时间：
1983-02
期刊：
影响因子：
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通讯作者：
H. Waldeland;J. K. Frenkel
中科院分区：
文献类型：
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作者：
H. Waldeland;J. K. Frenkel
用不同阶段的活生物体（即，速殖子、缓殖子或子孢子），或用有或无佐剂的杀死的速殖子。使用的佐剂是脂质体、肉豆蔻酸或月桂酸的酸酐、左旋咪唑和弗氏完全或不完全佐剂。对T.使用的弓形虫：RH，M-7741，不持久的温度敏感突变体ts-1、ts-4或ts-5，以及ts-1的“回复突变体”（Pfefferkorn和Pfefferkorn，1976）。通过用致病性M-7741菌株攻击来测量所提供的保护。单独或与佐剂一起杀死速殖子仅对M-7741攻击提供轻微保护，对所有对照小鼠致死的攻击剂量没有保护作用。慢性感染和非持久性活疫苗对攻击有很强的免疫力，但ts-1株除外。因为它的致病性较低，不需要化学预防，菌株ts-4最符合疫苗的要求;它在小鼠以外的宿主中的效果还有待确定。当用子孢子攻击时，速殖子、缓殖子或子孢子诱导的免疫力似乎同样强。
Mice were immunized with live organisms of the different stages (i.e., tachyzoites, bradyzoites, or sporozoites) of Toxoplasma gondii, or with killed tachyzoites with or without adjuvants. The adjuvants used were liposomes, anhydrides of myristic or lauric acid, levamisole and Freund's complete or incomplete adjuvant. The following strains of T. gondii were used: RH, M-7741, the nonpersisting, temperature-sensitive mutants ts-1, ts-4, or ts-5, and the "back mutant" of ts-1 (Pfefferkorn and Pfefferkorn, 1976). The protection afforded was measured by challenge with the pathogenic M-7741 strain. Killed tachyzoites alone, or with adjuvants, offered only slight protection against challenge with M-7741 and no protection against challenge doses that were lethal to all control mice. Chronic infection and live nonpersisting vaccines conveyed a strong immunity to challenge, except strain ts-1. Because it was less pathogenic and did not require chemoprophylaxis, strain ts-4 best fulfilled the requirements for a good vaccine; its effect in hosts other than the mouse remains to be determined. The immunity induced by tachyzoites, bradyzoites, or sporozoites appeared equally strong when challenged with sporozoites.