Quantitative autoradiography of multiple 5-HT1 receptor subtypes in the brain of control or 5,7-dihydroxytryptamine-treated rats
Quantitative autoradiography of multiple 5-HT1 receptor subtypes in the brain of control or 5,7-dihydroxytryptamine-treated rats
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对照或 5,7-二羟色胺治疗大鼠大脑中多种 5-HT1 受体亚型的定量放射自显影
DOI：
10.1523/jneurosci.06-12-03474.1986
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
M. Hamon
中科院分区：
文献类型：
--
作者：
D. Vergé;G. Daval;M. Marcinkiewicz;A. Patey;S. Mestikawy;H. Gozlan;M. Hamon
本文用光镜定量放射自显影法研究了大鼠脑内两种主要类型（A和B）5-HT_1结合位点的分布。用3 H-8-羟基-2-（N-二丙基氨基）四氢萘（3 H-8-OH-DPAT）或3 H-5-HT作为配体鉴定5-HT 1A位点。在后一种情况下，结果表明，3 H-5-HT与5-HT 1A位点的结合对应于被0.1 μ M 8-OH-DPAT或1 μ M螺哌隆置换的结合。在0.1 μ M 8-OH-DPAT存在下，3 H-5-HT标记的“非5-HT 1A”位点主要对应于5-HT 1B位点。5-HT 1A结合在边缘区（齿状回、CA 1和CA 3海马区、外侧隔、额叶皮质）中显著高，而5-HT 1B结合特别集中在锥体外系区（尾状核、苍白球、黑质）。除了在后者的地区，只有一类5-HT 1网站被发现，5-HT 1A和5-HT 1B网站存在于所有地区的审查。脑内注射5，7-二羟色胺所产生的肾上腺素能神经元的选择性变性仅与中缝背核的5-HT 1A结合（~ 60%）和黑质的5-HT 1B结合（~ 37%）的显著损失相关。这些结果进行了讨论有关的5-HT 1A和/或5-HT 1B网站与突触前5-HT自身受体控制神经冲动流和神经递质释放的肾上腺素能神经元的可能身份。
The distribution of the 2 main types (A and B) of 5-HT1 binding sites in the rat brain was studied by light-microscopic quantitative autoradiography. The 5-HT1A sites were identified using 3H-8-hydroxy-2- (N-dipropylamino)tetralin (3H-8-OH-DPAT) or 3H-5-HT as the ligand. In the latter case, it was shown that 3H-5-HT binding to 5-HT1A sites corresponded to that displaceable by 0.1 microM 8-OH-DPAT or 1 microM spiperone. The “non-5-HT1A” sites labeled by 3H-5-HT in the presence of 0.1 microM 8-OH-DPAT corresponded mainly to 5-HT1B sites. 5-HT1A binding was notably high in limbic regions (dentate gyrus, CA1 and CA3 hippocampal regions, lateral septum, frontal cortex), whereas 5-HT1B binding was particularly concentrated in extrapyramidal areas (caudate nucleus, globus pallidus, substantia nigra). Except in the latter regions, where only one class of 5-HT1 sites was found, both 5-HT1A and 5-HT1B sites existed in all areas examined. The selective degeneration of serotoninergic neurons produced by an intracerebral injection of 5,7- dihydroxytryptamine was associated only with a significant loss of 5- HT1A binding to the dorsal raphe nucleus (-60%) and of 5-HT1B binding to the substantia nigra (-37%). These results are discussed in relation to the possible identity of 5-HT1A and/or 5-HT1B sites with the presynaptic 5-HT autoreceptors controlling nerve impulse flow and neurotransmitter release in serotoninergic neurons.
血清素拮抗剂对大鼠血清素受体激活的两种行为模型的不同作用。
DOI：
--
发表时间：
1984
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Lucki,I;Nobler,MS;Frazer,A
通讯作者：
Frazer,A
测定大鼠额叶皮质中 5-HT 1A 和 5-HT 1B 受体亚型的选择性和非选择性化合物。
DOI：
--
发表时间：
1984
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Sills,MA;Wolfe,BB;Frazer,A
通讯作者：
Frazer,A
豚鼠海马中与腺苷酸环化酶连接的两个 5-HT 受体可被 5-羧酰胺色胺和螺哌隆识别。
影响因子：
5
作者：
Shenker,A;Maayani,S;Weinstein,H;Green,JP
通讯作者：
Green,JP