Controlled Release of Doxorubicin Loaded within Magnetic Thermo-responsive Nanocarriers under Magnetic and Thermal Actuation in a Microfluidic Channel
Controlled Release of Doxorubicin Loaded within Magnetic Thermo-responsive Nanocarriers under Magnetic and Thermal Actuation in a Microfluidic Channel
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DOI：
10.1021/nn3028425
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发表时间：
2012-12-01
期刊：
影响因子：
17.1
通讯作者：
Pellegrino, Teresa
中科院分区：
文献类型：
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作者：
Leal, Manuel Pernia;Torti, Andrea;Pellegrino, Teresa
我们报告了一种在磁性纳米载体表面生长温度响应性聚合物壳的方法，磁性纳米载体由多个氧化铁超顺磁性纳米颗粒嵌入聚（马来酸酐-alt-1-十八碳烯）聚合物纳米珠制成。根据共聚单体及其相对组成，可以实现在生理条件下在26至47摄氏度范围内的可调相变温度。使用合适的微流体平台结合磁性纳米结构和模仿循环系统毛细血管的通道，我们证明了热响应纳米珠适合于结合热和磁激活的局部药物递送。在临界温度以下，纳米珠在悬浮液中是稳定的，保留它们的货物，并且不容易被磁场捕获。将温度增加到临界温度以上会导致纳米珠聚集，形成具有足够高的磁矩的簇，以允许它们被靠近靶向区域的合适的磁梯度捕获。同时，聚合物溶胀激活药物释放，对于与毛细血管中的血液相同数量级的流速，特征时间大约为一小时。
We report a procedure to grow thermo-responsive polymer shells at the surface of magnetic nanocarriers made of multiple iron oxide superparamagnetic nanoparticles embedded in poly(maleic anhydride-alt-1-ocatadecene) polymer nanobeads. Depending on the comonomers and on their relative composition, tunable phase transition temperatures in the range between 26 and 47 degrees C under physiological conditions could be achieved. Using a suitable microfluidic platform combining magnetic nanostructures and channels mimicking capillaries of the circulatory system, we demonstrate that thermo-responsive nanobeads are suitable for localized drug delivery with combined thermal and magnetic activation. Below the critical temperature nanobeads are stable in suspension, retain their cargo, and cannot be easily trapped by magnetic fields. Increasing the temperature above the critical temperature causes the aggregation of nanobeads, forming clusters with a magnetic moment high enough to permit their capture by suitable magnetic gradients in close proximity to the targeted zone. At the same time the polymer swelling activates drug release, with characteristic times on the order of one hour for flow rates of the same order as those of blood in capillaries.