The miCube open microscope
The miCube open microscope
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miCube 开放式显微镜
DOI：
10.1038/s41592-019-0607-4
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发表时间：
2019
期刊：
影响因子：
48
通讯作者：
R. Strack
中科院分区：
文献类型：
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作者：
R. Strack
长读段测序技术在基因组组装、结构变异检测和单倍型定相方面具有优势，但由于与短读段测序相比错误率高，因此不太适合单核苷酸变异（SNV）和插入/缺失（indel）调用。Wenger等人，来自Pacific Biosciences的研究人员优化了环状共有序列测序（CCS）方案以实现长的高保真读段，其中他们选择了具有在15 kb处紧密分布的级分的SMRTbell文库用于高覆盖测序。CCS文库制备的灵感和优化基于他们的发现，即聚合酶在无损伤DNA分子上具有更好的存活率。他们采用CCS方案对人类基因组（HG 002）进行测序，平均长度为13.5 kb，平均准确率为99.8%。CCS性能与SNV和indel调用中的短读段测序的性能相当或更好。LT https：//doi. org/10.1038/s41592-019-0605-6 struCturaL BIoLogY移植抗螺旋抗体表位
Long-read sequencing technologies have advantages in genome assembly, structural variant detection and haplotype phasing, but are less suited for single-nucleotide variant (SNV) and insertion/deletion (indel) calling due to the high error rate in comparison with short-read sequencing. Wenger et al., from Pacific Biosciences, optimized the circular consensus sequencing (CCS) protocol to achieve long, high-fidelity reads, in which they selected the SMRTbell library with fractions tightly distributed at 15 kb for high-coverage sequencing. The CCS library preparation was inspired and optimized based on their findings that polymerases have a better survival on damage-free DNA molecules. They employed the CCS protocol in sequencing the human genome (HG002) and achieved an average length of 13.5 kb, and an average accuracy of 99.8%. The CCS performance is comparable to, or better than, that of short-read sequencing in SNV and indel calling. LT https://doi. org/10.1038/s41592-019-0605-6 struCturaL BIoLoGY transplanting antihelix antibody epitopes