A new episcopic method for rapid 3-D reconstruction:: applications in anatomy and embryology
A new episcopic method for rapid 3-D reconstruction:: applications in anatomy and embryology
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DOI：
10.1007/s004290050144
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发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Müller, GB
中科院分区：
文献类型：
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作者：
Weninger, WJ;Meng, S;Müller, GB
复杂结构的拓扑关系和器官系统的形态发生只有在三维背景下才能充分理解。物理切片标本的三维重建已成为现代解剖学和胚胎学研究中不可或缺的工具。教学也越来越多地使用三维表示，特别是在胚胎系统经历复杂的形式和形状变化的情况下。目前，没有廉价和简单的技术，可用于生成精确的三维模型的部分对象。在这项研究中，我们描述了一种新的技术，迅速提供忠实的3-D模型的切片标本。在每个切片和“块上”染色步骤之后，直接从包埋块的切割表面捕获图像。自动图像处理生成一堆样本轮廓的二进制图像。通过自动分割和手动跟踪两种方法获得内部结构的二值图像。由于这些图像系列是固有的对齐，它们可以被重建三维没有耗时的对齐过程。该方法的质量和灵活性通过重建三种不同的组织学组成和染色对比度的标本来证明：4 mm的小鼠胚胎连同其几个内脏器官，一个人类婴儿的海绵窦区域和一段人类颈动脉。非常短的处理时间和复杂的结构安排的忠实代表推荐这种技术在形态学研究和创建胚胎教学模型或3-D胚胎分期系列的常规使用。
The topographic relations of complex structures and the morphogenesis of organ systems can only be fully understood in their three-dimensional context. Three-dimensional (3-D) reconstruction of physically sectioned specimens has become an indispensable tool in modern anatomical and embryological research. Teaching also makes increasingly use of 3-D representations, in particular in the case of embryonic systems that undergo complicated transformations of form and shape. At present no cheap and simple technique is available that generates accurate 3-D models of sectioned objects. In this study we describe a novel technique that rapidly provides faithful 3-D models of sectioned specimens. The images are captured directly from the cutting surface of the embedding block after each sectioning and "on block" staining step. Automatic image processing generates a stack of binary images of the specimen contour. Binary images of internal structures are obtained both by automatic segmentation and manual tracing. Since these image series are inherently aligned, they can be reconstructed three-dimensionally without time-consuming alignment procedures. The quality and the flexibility of the method are demonstrated by reconstructing three kinds of specimens of different histological composition and staining contrast: a 4 mm mouse embryo together with several of its inner organs, a cavernous sinus region of a human infant, and a segment of a human carotid artery. Very short processing times and the faithful representation of complex structural arrangements recommend this technique for routine use in morphological research and for creating embryologic teaching models or 3-D embryonic staging series.