SPOT POSITION OF RAT-LIVER RIBOSOMAL-PROTEINS BY 4 DIFFERENT 2-DIMENSIONAL ELECTROPHORESES IN POLYACRYLAMIDE-GEL
SPOT POSITION OF RAT-LIVER RIBOSOMAL-PROTEINS BY 4 DIFFERENT 2-DIMENSIONAL ELECTROPHORESES IN POLYACRYLAMIDE-GEL
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DOI：
10.1007/bf00269998
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
REBOUD, JP
中科院分区：
文献类型：
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作者：
MADJAR, JJ;ARPIN, M;REBOUD, JP
采用4种不同的二维聚丙烯酰胺凝胶电泳系统分离大鼠肝脏40S和60S核糖体亚基和多体蛋白。第一个维度是在酸性或碱性pH下运行，第二个维度是在十二烷基硫酸钠或18%丙烯酰胺的酸性pH下运行。确定了每个系统中每个亚基和多体的单个蛋白质的位置。这一鉴定源于一种新的方法，避免了以往对单个蛋白质的纯化。新提出的哺乳动物核糖体蛋白统一命名法（McConkey et al. in press）用于对4种系统中的蛋白质进行编号。
Separation of the proteins from rat liver 40S and 60S ribosomal subunits and polysomes was done in 4 different 2-dimensional polyacrylamide gel electrophoresis systems. The 1st dimension was run at acidic or basic pH, the 2nd dimension either with sodium dodecyl sulfate or at acidic pH in 18% acrylamide. The position of each individual protein of both subunits and polysomes was determined in each system. This identification resulted from a new method avoiding any previous purification of individual proteins. The new proposed uniform nomenclature for mammalian ribosomal proteins (McConkey et al. in press) was used for numbering the proteins in the 4 systems.