Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development
Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development
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DOI：
10.3390/antibiotics9080456
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发表时间：
2020-08-01
影响因子：
4.8
通讯作者：
Lee, Yong Bok
中科院分区：
文献类型：
--
作者：
Cheong, Mi Sun;Seo, Kyung Hye;Lee, Yong Bok
兽用抗生素如磺胺类广泛用于提高饲料效率和预防畜牧生产中的疾病。磺胺类抗菌剂的抗菌机制涉及通过竞争性抑制细菌二氢蝶酸合酶（DHPS）活性来阻断叶酸的生物合成。有趣的是，大多数治疗抗生素可以通过粪便释放到环境中，并导致环境中的严重扩散污染。然而，磺胺在植物生长和发育过程中的生理效应仍然是难以捉摸的，因为植物的反应依赖于叶酸的生物合成和抗生素的浓度。在这里，我们提出了一个化学相互作用的对接模型之间的甘蓝（油菜）DHPS和磺胺甲恶唑和磺胺二甲嘧啶，这是最丰富的磺胺类药物在环境中检测到。此外，在磺酰胺暴露后，在扁豆（透镜culinaris）、水稻（Oryza sativa）和Napa卷心菜植物中观察到幼苗生长抑制。结果表明，磺胺类抗生素以类似于细菌DHPS的模块靶向植物DHPS，并影响作物幼苗的早期生长和发育。综合这些结果，我们认为磺胺类药物是农田中的污染物。
Veterinary antibiotics such as sulfonamides are widely used to increase feed efficiency and to protect against disease in livestock production. The sulfonamide antimicrobial mechanism involves the blocking of folate biosynthesis by inhibiting bacterial dihydropteroate synthase (DHPS) activity competitively. Interestingly, most treatment antibiotics can be released into the environment via manure and result in significant diffuse pollution in the environment. However, the physiological effects of sulfonamide during plant growth and development remain elusive because the plant response is dependent on folate biosynthesis and the concentration of antibiotics. Here, we present a chemical interaction docking model between Napa cabbage (Brassica campestris) DHPS and sulfamethoxazole and sulfamethazine, which are the most abundant sulfonamides detected in the environment. Furthermore, seedling growth inhibition was observed in lentil bean (Lens culinaris), rice (Oryza sativa), and Napa cabbage plants upon sulfonamide exposure. The results revealed that sulfonamide antibiotics target plant DHPS in a module similar to bacterial DHPS and affect early growth and the development of crop seedlings. Taking these results together, we suggest that sulfonamides act as pollutants in crop fields.