Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways
Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways
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DOI：
10.1021/acscatal.6b02979
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发表时间：
2017-01-01
期刊：
影响因子：
12.9
通讯作者：
Flitsch, Sabine L.
中科院分区：
文献类型：
--
作者：
France, Scott P.;Hepworth, Lorna J.;Flitsch, Sabine L.
通过非天然合成级联的连续生物催化反应的组合是一个快速发展的领域，并导致从简单的前体产生复杂的有价值的化学品。随着可用生物催化剂的工具箱不断扩大，靶分子的生物催化逆合成的选择也在增加，从而导致采用酶促转化的替代途径。这种级联反应的实施需要仔细考虑，特别是关于该途径是在体外还是在体内构建。在这个角度来看，我们描述了在体外，在体内，和混合的方法来建立生物催化级联和展示在该地区的最新发展的相对优点。我们还强调了影响纯酶或化学酶，一锅，多步途径的设计和实施的因素。
The combination of sequential biocatalytic reactions, via non-natural synthetic cascades, is a rapidly developing field and leads to the generation of complex valuable chemicals from simple precursors. As the toolbox of available biocatalysts continues to expand, so do the options for biocatalytic retrosynthesis of a target molecule, leading to alternative routes employing enzymatic transformations. The implementation of such cascade reactions requires careful consideration, particularly with respect to whether the pathway is constructed in vitro or in vivo. In this Perspective, we describe the relative merits of in vitro, in vivo, and hybrid approaches to building biocatalytic cascades and showcase recent developments in the area. We also highlight the factors that influence the design and implementation of purely enzymatic or chemoenzymatic, one-pot, multistep pathways.