Reactive oxygen metabolites relax the lamb ductus arteriosus by stimulating prostaglandin production.
Reactive oxygen metabolites relax the lamb ductus arteriosus by stimulating prostaglandin production.
复制标题
活性氧代谢物通过刺激前列腺素的产生来放松羔羊动脉导管。
DOI：
10.1161/01.res.64.1.1
复制
发表时间：
1989
影响因子：
20.1
通讯作者：
Mauray,F
中科院分区：
文献类型：
--
作者：
Clyman,RI;Saugstad,OD;Mauray,F
为了确定氧代谢产物是否会导致导管松弛，我们使用了从36只近足月羔羊中获得的胎儿导管环，并测量了次黄嘌呤和黄嘌呤氧化酶组合产生的氧代谢产物的影响。由次黄嘌呤加黄嘌呤氧化酶产生的氧代谢产物引起导管松弛，其被过氧化氢酶（过氧化氢清除剂）抑制，但不被超氧化物歧化酶（超氧阴离子清除剂）抑制。此外，次黄嘌呤加黄嘌呤氧化酶使前列腺素（PG）E2的产量增加14倍，而6-酮-PGF 1 α（PGI 2的稳定代谢产物）仅增加2倍。前列腺素E_2是最有效的动脉导管舒张剂。过氧化氢酶或吲哚美辛的存在下，阻止前列腺素的生产和松弛的增加。我们的结论是，活性氧代谢物放松动脉导管，反对出生后发生的正常收缩。然而，导管中活性氧代谢产物的血管活性作用似乎仅通过PGE 2的产生介导。
To determine whether oxygen metabolites can cause ductus relaxation, we used rings of fetal ductus obtained from 36 near-term lambs and measured the effects of the oxygen metabolites generated by the combination of hypoxanthine and xanthine oxidase. The oxygen metabolites produced by hypoxanthine plus xanthine oxidase caused relaxation of the ductus that was inhibited by catalase (hydrogen peroxide scavenger) but not by superoxide dismutase (superoxide anion scavenger). In addition, hypoxanthine plus xanthine oxidase produced a 14-fold increase in prostaglandin (PG) E2 production with only twofold increase in 6-keto-PGF1 alpha (the stable metabolite of PGI2). PGE2 is the most potent relaxant of the ductus arteriosus. The presence of either catalase or indomethacin blocked both the increase in prostaglandin production and the relaxation. We conclude that reactive oxygen metabolites relax the ductus arteriosus and oppose the normal constriction that occurs after birth. However, the vasoactive effects of reactive oxygen metabolites in the ductus appear to be mediated exclusively through the generation of PGE2.