Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment
Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment
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DOI：
10.1016/j.msec.2012.07.039
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发表时间：
2012-12-01
影响因子：
7.9
通讯作者：
Ito, Yoshihiro
中科院分区：
文献类型：
--
作者：
Kang, Jeonghwa;Sakuragi, Makoto;Ito, Yoshihiro
用多巴胺修饰钛和不锈钢，固定化生物分子表皮生长因子（EGF）。首先，研究了多巴胺溶液在不同pH条件下对金属表面的处理。在较高的pH值下，多巴胺溶液变成棕色并形成沉淀物。在pH为8.5的条件下，多巴胺处理金属也导致金属表面呈现棕色。多巴胺处理后，表面的疏水性增加，与pH无关。x射线光电子能谱显示，pH为8.5时，表面上形成了大量的有机层。根据椭偏测量，在pH 8.5下形成的有机层厚度大约是pH 4.5下形成的有机层的1000倍。pH为8.5时形成的层中氨基酸的数量也高于pH为4.5时形成的层。通过碳二亚胺偶联反应将EGF分子固定在多巴胺处理过的表面上。与pH值为4.5的表面相比，在pH值为8.5的表面上固定了更多的EGF。在EGF存在的情况下，与未固定的表面相比，在两种EGF固定的表面上观察到的大鼠成纤维细胞的生长明显更高。本研究表明，金属可以通过表面固定化生长因子获得生物活性，并且固定化生长因子对金属的影响大于可溶性生长因子。(C) 2012 Elsevier B.V.版权所有
Titanium and stainless steel were modified with dopamine for the immobilization of biomolecules, epidermal growth factor (EGF). First, the treatment of metal surfaces with a dopamine solution under different pH conditions was investigated. At higher pH, the dopamine solution turned brown and formed precipitates. Treatment of the metals with dopamine at pH 8.5 also resulted in the development of brown color at the surface of the metals. The hydrophobicity of the surfaces increased after treatment with dopamine, independently of pH. X-ray photoelectron spectroscopy revealed the formation of a significant amount of an organic layer on both surfaces at pH 8.5. According to ellipsometry measurements, the organic layer formed at pH 8.5 was about 1000 times as thick as that formed at pH 4.5. The amount of amino groups in the layer formed at pH 8.5 was also higher than that observed in the layer formed at pH 4.5. EGF molecules were immobilized onto the dopamine-treated surfaces via a coupling reaction using carbodiimide. A greater amount of EGF was immobilized on surfaces treated at pH 8.5 compared with pH 4.5. Significantly higher growth of rat fibroblast cells was observed on the two EGF-immobilized surfaces compared with non-immobilized surfaces in the presence of EGF. The present study demonstrated that metals can become bioactive via the surface immobilization of a growth factor and that the effect of the immobilized growth factor on metals was greater than that of soluble growth factor. (C) 2012 Elsevier B.V. All rights reserved.