HUMAN DISTRIBUTION AND RELEASE OF A PUTATIVE NEW GUT HORMONE, PEPTIDE-YY
HUMAN DISTRIBUTION AND RELEASE OF A PUTATIVE NEW GUT HORMONE, PEPTIDE-YY
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DOI：
10.1016/0016-5085(85)90211-2
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发表时间：
1985-01-01
期刊：
影响因子：
29.4
通讯作者：
BLOOM, SR
中科院分区：
文献类型：
--
作者：
ADRIAN, TE;FERRI, GL;BLOOM, SR
建立了一种新的肠道激素多肽酪氨酸[YY(PYY)]的放射免疫分析方法。在人类胃肠道的不同层中测量了多肽YY的浓度，其中PYY仅在含有内分泌细胞的粘膜上皮中发现。YY肽分布于整个小肠，在十二指肠(6pmol/g)和空肠(5pmoL/g)中含量很低，而在回肠末端含量较高(84pmoL/g)。乙状结肠和乙状结肠的浓度均较高，分别为82pmo1/g和196pmo1/g，直肠内浓度最高(480pmol/g)。经凝胶渗透层析和反相高压液相色谱鉴定，人肠道中PYY样免疫反应的主要分子形式似乎与纯猪激素相同。PYY的基础血浆浓度很低，但随着食物的进食而升高，餐后几个小时保持在较高水平。已知的PYY强大的生物学作用，其在肠道内分泌细胞中的高浓度，以及它在正常饮食后释放到循环中，表明该肽可能在生理上作为一种循环肠道激素发挥作用。
A radioimmunoassay has been developed for the new intestinal hormonal peptide tyrosine tyrosine [peptide YY (PYY)]. Peptide YY concentrations were measured in separated layers of the human gastrointestinal tract, where PYY was found exclusively in the mucosal epithelium which contained the endocrine cells. Peptide YY was found throughout the small intestine, in very low concentrations (5 pmol/g) in duodenum (6 pmol/g) and jejunum (5 pmol/g), but in higher concentrations in the terminal ileum (84 pmol/g). High concentrations were found throughout the colon (ascending 82 pmol/g, sigmoid 196 pmol/g), being maximum in the rectum (480 pmol/g). The major molecular form of PYY-like immunoreactivity in human intestine appeared to be identical to pure porcine hormone, both as judged by gel permeation chromatography and by reverse-phase high-pressure liquid chromatography. Basal plasma concentrations of PYY were low but rose in response to food, remaining elevated for several hours postprandially. The known potent biologic actions of PYY, its high concentrations in gut endocrine cells, and its release into the circulation after a normal meal suggest that this peptide may function physiologically as a circulating gut hormone.