Variation in antigenic determinants of p53 transformation-related protein obtained from various species.
Variation in antigenic determinants of p53 transformation-related protein obtained from various species.
复制标题
从不同物种获得的 p53 转化相关蛋白的抗原决定簇的变化。
作者：
V. Rotter;H. Friedman;A. Katz;K. Zerivitz;D. Wolf
p53是细胞编码的转化相关蛋白。它在肿瘤细胞中的合成水平升高，但在几种类型的非转化细胞中也检测到低浓度。肿瘤细胞的p53是免疫原性的，并且产生特异性抗体。通过与从RA 3 - 2C 2、PAb 122和PAb 421建立的杂交瘤细胞系获得的抗p53单克隆抗体特异性结合，研究了p53蛋白的抗原决定簇，并在各种动物物种中进行了保护。我们发现，小鼠p53与所有三种抗p53单克隆抗体均有效免疫沉淀，而人和大鼠p53与PAb 122和PAb 421结合，但缺乏结合RA 3 - 2C 2的决定簇。仓鼠p53分子代表第三类，其与多克隆抗p53抗体免疫沉淀，但未能结合所有三种单克隆抗体分析。使用这些单克隆抗体，我们检测到p53在转化和p53在非转化细胞中发现，在一个给定的物种之间没有变化。结果还表明，识别小鼠特异性决定簇的RA 3 - 2C 2结合位于p53分子的蛋白水解消化片段上的位点，该位点不同于含有PAb 122和PAb 421识别位点的位点。p53是一种单一的蛋白质，可以通过不同的抗原决定簇免疫沉淀，这些抗原决定簇在物种之间是不同的。
p53 is a cellular-encoded transformation-related protein. It is synthesized at elevated levels in tumor cells but has also been detected at low concentrations in several types of nontransformed cells. The p53 of tumor cells is immunogenic and elicits specific antibody production. The antigenic determinants of the p53 protein were studied by specific binding to anti-p53 monoclonal antibodies obtained from the RA3-2C2, PAb122, and PAb421 established hybridoma cell lines, and their conservation was followed in various animal species. We found that whereas mouse p53 efficiently immunoprecipitated with all three anti-p53 monoclonal antibodies, human and rat p53 bound PAb122 and PAb421 but lacked a determinant binding RA3-2C2. The hamster p53 molecule represented a third category, which immunoprecipitated with polyclonal anti-p53 antibodies but failed to bind all three monoclonal antibodies analyzed here. Using these monoclonal antibodies, we detected no variations between p53 found in transformed and p53 found in nontransformed cells, within a given species. The results also showed that RA3-2C2, which recognizes a mouse-specific determinant, binds a site located at a proteolytic digestion fragment of the p53 molecule that differs from that containing PAb122 and PAb421 recognition site(s). p53 is a single protein that can be immunoprecipitated through different antigenic determinants that vary between species.