High performance liquid chromatographic methods for the determination of aripiprazole with ultraviolet detection in rat plasma and brain: Application to the pharmacokinetic study
High performance liquid chromatographic methods for the determination of aripiprazole with ultraviolet detection in rat plasma and brain: Application to the pharmacokinetic study
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DOI：
10.1016/j.jchromb.2005.03.024
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发表时间：
2005-07-05
影响因子：
3
通讯作者：
Miyamoto, G
中科院分区：
文献类型：
--
作者：
Shimokawa, Y;Akiyama, H;Miyamoto, G
对测定大鼠血浆和脑中阿立哌唑（OPC- 14597，Ability（TM））的高效液相色谱（HPLC）方法进行了验证。色谱柱为Nova-pak苯基柱，移动的相为乙腈-甲醇-20 mM硫酸钠-乙酸（27：25：48：1，v/v/v/v），流速为1.0 ml/min，紫外检测波长为254 nm。血浆和脑中的重现性显示出良好的精密度（在7.8和10.6%范围内）和准确度（96.0-102.4%和99.0-108.7%），校准曲线范围分别为10.0-2000 ng/ml和30.0-6000 ng/g。经验证的HPLC方法成功应用于大鼠阿立哌唑的药代动力学研究，证明口服给药后的脑浓度比血浆浓度高5倍。(c)2005 Elsevier B. V.保留所有权利。
High performance liquid chromatographic (HPLC) methods were validated for the determination of aripiprazole (OPC- 14597, Ability (TM)) in rat plasma and brain. Separation was by Nova-pak phenyl column; flow rate, 1.0 ml/min; mobile phase, acetonitrile-methanol-20 mM sodium sulfate-acetic acid (27:25:48: 1, v/v/v/v); UV detection at 254 nm. Reproducibility in plasma and brain showed excellent precision (within 7.8 and 10.6%) and accuracy (96.0-102.4% and 99.0-108.7%) with calibration curve ranges 10.0-2000 ng/ml and 30.0-6000 ng/g, respectively. Validated HPLC methods were successfully applied to pharmacokinetic study of aripiprazole in rats, demonstrating brain concentrations after oral administration five times higher than plasma concentrations. (c) 2005 Elsevier B.V. All rights reserved.