Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing.
Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing.
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牛津纳米孔R10.4长读测序能够从纯培养物和元基因组中产生接近完成的细菌基因组，而不需要短读或参考抛光。
DOI：
10.1038/s41592-022-01539-7
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发表时间：
2022-07
期刊：
影响因子：
48
通讯作者：
Albertsen M
中科院分区：
文献类型：
--
作者：
Sereika M;Kirkegaard RH;Karst SM;Michaelsen TY;Sørensen EA;Wollenberg RD;Albertsen M
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing. This study demonstrates the feasibility of generating near-finished microbial genomes using only Oxford Nanopore R10.4 data from pure cultures or metagenomes.
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