Modeling neural circuit, blood-brain barrier, and myelination on a microfluidic 96 well plate
Modeling neural circuit, blood-brain barrier, and myelination on a microfluidic 96 well plate
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DOI：
10.1088/1758-5090/ab1402
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发表时间：
2019-07-01
期刊：
影响因子：
9
通讯作者：
Jeon, Noo Li
中科院分区：
文献类型：
--
作者：
Lee, Seung-Ryeol;Hyung, Sujin;Jeon, Noo Li
微流体使神经科学领域的各种实验成为可能。不幸的是，由于固有的材料兼容性问题和缺乏标准化的可制造设备，基于聚二甲基硅氧烷(PDMS)的微流控器件的更广泛采用面临着挑战。在这项工作中，我们提出了一种注塑塑料阵列三维(3D)神经元培养平台(Neuro-Impact)，由聚苯乙烯(PS)制成，具有标准的96孔板形状因数，可以概括中枢和周围神经系统的元素。标准化的神经元体外培养平台将促进神经退行性疾病新疗法的开发，因为它们将使基于成像和生化分析的定量分析成为可能。为了证明Neuro-Impact的多功能性，我们模拟了生理上相关的复杂共培养模型，如3D神经元网络、血脑屏障和髓鞘形成。Neuro-Impact提供了一个高通量的筛查兼容平台，具有设计神经元微环境的能力，以帮助基础和应用神经科学研究。
Microfluidics have enabled a wide range of experimental possibilities in the field of neuroscience. Unfortunately, the wider scale adoption of polydimethylsiloxane (PDMS) based microfluidic devices faces challenges due to inherent material compatibility issues and lack of standardized manufacturable devices. In this work, we present an injection molded plastic array three-dimensional (3D) neuron culture platform (Neuro-IMPACT) made of polystyrene (PS) with a standard 96-well plate form factor that can recapitulate elements of both the central and peripheral nervous systems. A standardized in vitro platform for neuron culture will facilitate the development of new therapies for neurodegenerative diseases, as they would enable quantitative analysis based on imaging as well as biochemical analysis. To demonstrate the versatility of Neuro-IMPACT, we modeled physiologically relevant complex co-culture models such as a 3D neuronal network, blood-brain barrier, and myelination. The Neuro-IMPACT offers a high-throughput screening compatible platform with the ability to engineer the neuronal microenvironment to aid both basic and applied neuroscience research.