A protocol to analyse cellular dynamics during plant development
A protocol to analyse cellular dynamics during plant development
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DOI：
10.1111/j.1365-313x.2005.02576.x
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发表时间：
2005-12-01
期刊：
影响因子：
7.2
通讯作者：
Traas, J
中科院分区：
文献类型：
--
作者：
de Reuille, PB;Bohn-Courseau, I;Traas, J
体内显微镜生成的图像包含三维（3D）物体动态行为的复杂信息。因此，需要适当的数学和计算工具来帮助解释。理想情况下，一个完整的软件链，以研究一个复杂的三维物体的动态应该包括：（i）采集，（ii）预处理和（iii）分割的图像，其次是（iv）重建的时间和空间和（v）最终的定量分析。在这里，我们已经开发了这样一个协议来研究细胞动力学在芽顶端分生组织在拟南芥。该方案使用用共聚焦显微镜制作的连续光学切片。它包括专门设计的算法来自动识别细胞谱系和分析分生组织表面的定量行为。
In vivo microscopy generates images that contain complex information on the dynamic behaviour of three-dimensional (3D) objects. As a result, adapted mathematical and computational tools are required to help in their interpretation. Ideally, a complete software chain to study the dynamics of a complex 3D object should include: (i) the acquisition, (ii) the preprocessing and (iii) segmentation of the images, followed by (iv) a reconstruction in time and space and (v) the final quantitative analysis. Here, we have developed such a protocol to study cell dynamics at the shoot apical meristem in Arabidopsis. The protocol uses serial optical sections made with the confocal microscope. It includes specially designed algorithms to automate the identification of cell lineage and to analyse the quantitative behaviour of the meristem surface.