Vitreous microparticles contain apoptotic signals suggesting a diabetic vitreopathy.
Vitreous microparticles contain apoptotic signals suggesting a diabetic vitreopathy.
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DOI：
10.18240/ijo.2022.01.14
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发表时间：
2022-01
影响因子：
1.4
通讯作者：
Harris Sultan;R. Rajagopal;P. Rao;Kisha Piggott;Michael A. Paley;L. Hassman;A. S. Li;B. Marshall;R. Apte
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作者：
Harris Sultan;R. Rajagopal;P. Rao;Kisha Piggott;Michael A. Paley;L. Hassman;A. S. Li;B. Marshall;R. Apte
目的比较糖尿病患者与非糖尿病患者玻璃体切割术中玻璃体液中微粒分布的差异。方法对34眼施行玻璃体切除手术的患者行无遮盖横切术。收集玻璃体标本并进行处理，以评估膜完整性(DAPI)、细胞凋亡率(Annexin-V)和内皮细胞来源(V-钙粘附素)。使用BD LSR II流式细胞仪进行分析，并使用标准化的亚微米尺寸的珠子进行大小比较。结果共检测出34例标本。有血进入玻璃体的微粒Annexin-V水平(n=12)高于无血的微粒(52.3%±30.7%vs19.6%±27.2%，P=0.002)。糖尿病手术并出血组(n=7)Annexin-V的表达高于未出血组(62.1%±31.7%vs18.9%±20.9%，P=0.009)。而在非糖尿病玻璃体出血患者中，Annexin-V的表达水平与其他疾病进程相比差异无统计学意义(38.6%±25.7%，n=5 vs20.0%±30.9%，n=0.087)。结论糖尿病玻璃体出血玻璃体微粒上可检测到细胞凋亡标记物Annexin-V的高表达。在评估非糖尿病患者的玻璃体出血时，细胞凋亡信号并无明显不同。糖尿病患者玻璃体切除术和视力的改善，可能与去除血清来源的、促细胞凋亡的玻璃体有关。为了确定调控疾病的微粒的分子特征，有必要进行进一步的研究。
AIM To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing vitrectomy. METHODS Un-masked cross-sectional series of 34 eyes undergoing vitrectomy. Vitreous specimens were collected and processed to evaluate for membrane integrity (DAPI), apoptosis (Annexin-V), and endothelial-cell origin (V-Cadherin). A BD LSR II flow cytometer was used for analysis and standardized sub-micron-sized beads were used for size comparison. RESULTS Thirty-four specimens underwent analysis. Greater levels of Annexin-V were found on microparticles from specimens in which blood had entered the vitreous (n=12) compared to those without blood (n=22; 52.3%±30.7% vs 19.6%±27.2%, P=0.002). Patients with diabetes having surgery with hemorrhage (n=7) had greater expression of Annexin-V than those without hemorrhage (n=8; 62.1%±31.7% vs 18.9%±20.9%, P=0.009). However, in patients with non-diabetic vitreous hemorrhage, the level of Annexin-V expression was not significantly different compared to other disease processes (38.6%±25.7%, n=5 vs 20.0%±30.9%, n=14, P=0.087). CONCLUSION Increased expression of the apoptotic marker, Annexin-V is detected on vitreous microparticles in diabetes-related vitreous hemorrhage. When evaluating vitreous hemorrhage in patients without diabetes, the apoptotic signal is not significantly different. Vitrectomy in patients with diabetes, and improvement in visual outcomes, may be related to the removal of a serum-derived, pro-apoptotic vitreous. Further investigation is warranted in order to identify the molecular characteristics of microparticles that regulate disease.