Intermittency properties in a temporal lobe epilepsy model
Intermittency properties in a temporal lobe epilepsy model
复制标题
DOI：
10.1016/j.yebeh.2022.109072
复制
发表时间：
2023-01-16
影响因子：
2.6
通讯作者：
Kihara, A. H.
中科院分区：
文献类型：
--
作者：
Borges, F. S.;Gabrickc, E. C.;Kihara, A. H.
神经元同步对于大脑区域之间的交流非常重要，在学习中起着关键作用。然而，连通性的改变可能导致与癫痫发作相关的超同步状态，这种状态与异步状态间歇性发生。活性调节的细胞骨架相关蛋白（ARC）与可导致癫痫的突触改变有关。在啮齿类动物模型中，诱导癫痫持续状态导致出现强烈的ARC免疫反应神经元（IAINs），其连接数量和电导强度高于非IAINs。这种改变可能导致异常的癫痫发作活动。在这项工作中，我们研究了IAINs连接如何影响神经网络的放电模式和同步。首先，由于iain的出现，我们展示了同步爆发模式的出现。其次，我们描述了iain连接的增加如何分别与同步突发和异步峰值相关的间歇性上下活动的出现。一旦间歇性活性得到适当的表征，我们应用光遗传学控制在间歇性状态下的高同步活性。为了做到这一点，我们认为1%的神经元被转染并变得光敏。我们观察到光遗传学方法控制同步爆发模式是有效的，当IAINs被选择为光敏的，而不是有效的非IAINs。因此，我们的分析表明，IAINs在高度同步活动的产生和抑制中都起着关键作用。(c) 2022爱思唯尔公司版权所有。
Neuronal synchronization is important for communication between brain regions and plays a key role in learning. However, changes in connectivity can lead to hyper-synchronized states related to epileptic sei-zures that occur intermittently with asynchronous states. The activity-regulated cytoskeleton-associated protein (ARC) is related to synaptic alterations which can lead to epilepsy. Induction of status epilepticus in rodent models causes the appearance of intense ARC immunoreactive neurons (IAINs), which present a higher number of connections and conductance intensity than non-IAINs. This alteration might con-tribute to abnormal epileptic seizure activity. In this work, we investigated how IAINs connectivity influ-ences the firing pattern and synchronization in neural networks. Firstly, we showed the appearance of synchronized burst patterns due to the emergence of IAINs. Second, we described how the increase of IAINs connectivity favors the appearance of intermittent up and down activities associated with syn-chronous bursts and asynchronous spikes, respectively. Once the intermittent activity was properly char-acterized, we applied the optogenetics control of the high synchronous activities in the intermittent regime. To do this, we considered that 1% of neurons were transfected and became photosensitive. We observed that optogenetics methods to control synchronized burst patterns are effective when IAINs are chosen as photosensitive, but not effective in non-IAINs. Therefore, our analyses suggest that IAINs play a pivotal role in both the generation and suppression of highly synchronized activities.(c) 2022 Elsevier Inc. All rights reserved.