2,3-Dihydrofarnesoic acid, a unique terpene from trichomes ofLycopersicon hirsutum, repels spider mites
2,3-Dihydrofarnesoic acid, a unique terpene from trichomes ofLycopersicon hirsutum, repels spider mites
复制标题
2,3-二氢金合欢酸是一种来自番茄毛状体的独特萜烯，可驱除红蜘蛛
DOI：
--
复制
发表时间：
1993
影响因子：
2.3
通讯作者：
J. Pyrek
中科院分区：
文献类型：
--
作者：
J. Snyder;Zhenhua Guo;R. Thacker;J. Goodman;J. Pyrek
hirsutum是番茄的野生近亲，对节肢动物食草动物具有高度抗性。两种植物形式的l。hirsutum, l.h hirsutum f.g abratum （C.H. Mull.）和l.h。典型毛癣（Humb）& Bonpl.)，对双斑蜘蛛螨，荨麻疹叶螨具有抗性。然而，典型赤霉病的叶片和毛状分泌物对螨虫的驱避作用比光毛赤霉病强。我们之前已经证明，典型假单胞菌LA 1363和LA 1927的毛状分泌物可以驱螨。在本文中，我们报告了鉴别的主要成分的毛分泌物负责驱避。收集具有类似于毛状分泌物成分的小叶洗涤液并将其分离成中性和酸性组分；驱避作用主要与酸部分有关，当酸部分作用于无驱避作用的光秃秃小叶时，使其具有驱避作用。用高效液相色谱法分离小叶洗涤液，然后用气相色谱-质谱联用和核磁共振法对2,3-二氢famesoic酸（3,7,11-三甲基- 6,10 -十二烯酸）作为主要的驱避化学成分进行纯化和鉴定。这种酸在l小叶上的应用。鳞片使它们令人反感。酸馏分中还含有少量的法脂酸和16∶0、16∶3、18∶0、18∶2和18∶3脂肪酸。这是首次报道天然存在的2,3-二氢法尼松酸。
Lycopersicon hirsutum, a wild relative of the tomato, is highly resistant to arthropod herbivores. Both botanic forms ofL. hirsutum, L. hirsutum f.glabratum (C.H. Mull.) andL. hirsutum f.typicum (Humb. & Bonpl.), are resistant to two-spotted spider mites,Tetranychus urticae Koch. However, leaves and trichome secretions from f.typicum repel mites more so than those from f.glabratum. We have previously demonstrated that trichome secretions from LA 1363 and LA 1927, accessions of f.typicum, repelled mites. In this paper we report the identification of the primary component of trichome secretions responsible for repellency. Leaflet washes having compositions similar to trichome secretions were collected and separated into neutral and acid fractions; repellency was mainly associated with the acid fraction, which, when applied to nonrepellent leaflets of f.glabratum, rendered them repellent. Separation of leaflet washes by HPLC allowed purification and subsequent identification by gas chromatography-mass spectrometry and nuclear magnetic resonance of 2,3-dihydrofamesoic acid (3,7,11-trimethyl-6, 10-dodecadienoic acid) as the primary chemical component responsible for repellency. Application of this acid to leaflets ofL. esculentum rendered them repellent. Other volatile compounds present in minor amounts in the acid fractions were farnesoic acid and 16∶0, 16∶3, 18∶0, 18∶2, and 18∶3 fatty acids. This is the first report of the natural occurrence of 2,3-dihydrofarnesoic acid.