The mystery of the trichothecene 3-O-acetyltransferase gene -: Analysis of the region around Tri101 and characterization of its homologue from Fusarium sporotrichioides
The mystery of the trichothecene 3-O-acetyltransferase gene -: Analysis of the region around Tri101 and characterization of its homologue from Fusarium sporotrichioides
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DOI：
10.1016/s0014-5793(98)01061-8
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发表时间：
1998-09-18
期刊：
影响因子：
3.5
通讯作者：
Yamaguchi, I
中科院分区：
文献类型：
--
作者：
Kimura, M;Matsumoto, G;Yamaguchi, I
毛霉烯3-O-乙酰转移酶基因Tr101对B型毛霉烯产生菌禾谷镰刀菌的生长起着关键作用。我们对含有Tri101的粘粒进行了分析，发现该抗性基因不在迄今报道的生物合成基因簇中。它可能位于与毛霉烯生物合成无关的UTP-氨连接酶基因和磷酸渗透酶基因之间，这两个看家基因在不产生毛霉烯的镰刀菌中也是连锁的。结果表明，Tri101的孤立发生归因于水平基因转移，而不是包含基因簇的染色体的相互易位。有趣的是，3-O-乙酰化并不总是所有t型三茂铁产生菌的主要自卫策略，即A型三茂铁产生菌不能在体内乙酰化T-2毒素，尽管该真菌具有功能上的3-O-乙酰基转移酶基因。因此，Tri101似乎是生产者在原有抵抗机制之外独立获得的一种防御选择。(C)1998年欧洲生化学会联合会。
The trichothecene 3-O-acetyltransferase gene, Tr101, plays a pivotal role for the well-being of the type B trichothecene producer Fusarium graminearum. We have analyzed the cosmids containing Tri101 and found that this resistance gene is not in the biosynthetic gene cluster reported so far. It mas located between the UTP-ammonia ligase gene and the phosphate permease gene which are not related to trichothecene biosynthesis, These two 'house-keeping' genes were also linked in Fusarium species that do not produce trichothecenes. The result suggests that the isolated occurrence of Tri101 is attributed to horizontal gene transfer and not to the reciprocal translocation of the chromosome containing the gene cluster. Interestingly, 3-O-acetylation mas not always a primary self-defensive strategy for all the t-type trichothecene producers; i,e. the type A trichothecene producer Fusarium sporotrichioides did not acetylate T-2 toxin in vivo although the fungus possessed a functional 3-O-acetyltransferase gene. Thus Tri101 appears to be a defense option which the producers have independently acquired in addition to their original resistance mechanisms. (C) 1998 Federation of European Biochemical Societies.