Lack of effect of recombinant human growth hormone (GH) on muscle morphology and GH-insulin-like growth factor expression in resistance-trained elderly men.
Lack of effect of recombinant human growth hormone (GH) on muscle morphology and GH-insulin-like growth factor expression in resistance-trained elderly men.
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DOI：
10.1210/jcem.81.1.8550787
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Dennis;-R.;Taaffe;Isabella;-H.;Jin;Andrew;Hoffman;Robert;Marcus
中科院分区：
文献类型：
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作者：
Dennis;-R.;Taaffe;Isabella;-H.;Jin;Andrew;Hoffman;Robert;Marcus
股外侧肌肌肉样品通过针吸活检从18名健康老年男性（65-82岁）获得，所述18名健康老年男性参与重组人GH（rhGH）和运动的双盲、安慰剂（PL）对照试验，并评估肌肉形态和骨骼肌组织中GH和胰岛素样生长因子（IGF）的表达。受试者最初接受14周的渐进式阻力训练，然后随机接受rhGH（0.02 mg/kg BW.day，sc）或PL，同时进行另外10周的训练。在基线和14周和24周时获得肌肉样品。I型和II型纤维的平均（+/- SEM）横截面积在基线时相似（I型，3891 +/- 167微米2; II型，3985 +/- 200微米2），在最初的14周训练期后分别增加（P < 0.01）16.2 +/- 4.1%和11.8 +/-3.8%。治疗后（第14-24周），双向重复测量ANOVA显示I型（P < 0.01）和II型纤维（P < 0.05）的时间主要影响，但无组效应或相互作用。PL组I型纤维（11.5 +/- 3.6%）的横截面积增加显著（P = 0.01），II型纤维（11.1 +/- 5.6%）的横截面积增加接近显著（P = 0.06）。对于rhGH，I型（6.3 +/- 5.9%）和II型（7.1 +/- 5.2%）纤维面积的变化不显著。在14周预处理期后或rhGH或PL治疗后，由于运动，组织GH受体、IGF-I、IGF-I受体、IGF-II或IGF-II受体信使核糖核酸未发生明显变化。这些结果表明，在行使老年男性rhGH管理不增加肌纤维肥大或组织GH-IGF的表达，并表明GH-IGF-I轴与老化的赤字不抑制骨骼肌组织对训练的反应。
Vastus lateralis muscle samples were obtained by needle biopsy from 18 healthy elderly men (65-82 yr) participating in a double blind, placebo (PL)-controlled trial of recombinant human GH (rhGH) and exercise and assessed for muscle morphology and skeletal muscle tissue expression of GH and insulin-like growth factors (IGFs). Subjects initially underwent progressive resistance training for 14 weeks and were then randomized to receive either rhGH (0.02 mg/kg BW.day, sc) or PL while undertaking a further 10 weeks of training. Muscle samples were obtained at baseline and at 14 and 24 weeks. The mean (+/- SEM) cross-sectional areas of type I and II fibers were similar (type I, 3891 +/- 167 microns2; type II, 3985 +/- 200 microns2) at baseline and increased (P < 0.01) by 16.2 +/- 4.1% and 11.8 +/- 3.8%, respectively, after the initial 14-week training period. After treatment (weeks 14-24), two-way repeated measures ANOVA revealed a main effect of time for type I (P < 0.01) and type II fibers (P < 0.05), but no group effect or interaction. The increase in cross-sectional area for the PL group was significant (P = 0.01) for type I (11.5 +/- 3.6%) and approached significance (P = 0.06) for type II fibers (11.1 +/- 5.6%). For rhGH, the change in type I (6.3 +/- 5.9%) and II (7.1 +/- 5.2%) fiber area was not significant. No apparent change in tissue GH receptor, IGF-I, IGF-I receptor, IGF-II, or IGF-II receptor messenger ribonucleic acids occurred as a result of exercise after the 14-week pretreatment period or after treatment with rhGH or PL. These results indicate that rhGH administration in exercising elderly men does not augment muscle fiber hypertrophy or tissue GH-IGF expression and suggests that deficits in the GH-IGF-I axis with aging do not inhibit the skeletal muscle tissue response to training.