The Inhibitory Effects of a Monoclonal Antibody Targeting Neuropilin-1 on Adhesion of Glioma Cells to Fibronectin
The Inhibitory Effects of a Monoclonal Antibody Targeting Neuropilin-1 on Adhesion of Glioma Cells to Fibronectin
复制标题
靶向 Neuropilin-1 的单克隆抗体对胶质瘤细胞与纤连蛋白粘附的抑制作用
DOI：
10.1166/jbn.2014.1867
复制
发表时间：
2014-11-01
影响因子：
2.9
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
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作者：
Chen, Lukui;Miao, Wei;Xu, Qiang
我们研究了抗neuropilin -1单克隆抗体（nlp -1 MAb）对胶质瘤细胞对纤维连接蛋白（FN）粘附的抑制作用及其相关机制。采用黏附实验和共聚焦荧光显微镜研究了NRP-1 MAb对U251胶质瘤细胞黏附和应力纤维形成的影响。用NRP-1 MAb处理U251胶质瘤细胞后，采用共免疫沉淀和Western blot检测FN粘附相关蛋白的变化。粘附实验显示，NRP-1 MAb对U251胶质瘤细胞的粘附有抑制作用。共聚焦荧光显微镜、共免疫沉淀和Western blot结果显示，NRP-1 MAb可以抑制粘附在U251细胞上的应力纤维的形成。NRP-1 MAb还可以影响Integrin α 5 β 1-NRP-1复合物的形成，并降低FAK和p130cas的磷酸化水平。由此可见，NRP-1 MAb对胶质瘤细胞粘附FN具有抑制作用。这种作用与NRP-1 MAb抑制Integrin α 5 β 1-NRP-1复合物的形成，从而抑制FAK/p130cas信号通路有关。
We investigated the inhibitory effects of anti-Neuropilin-1 monoclonal antibody (NRP-1 MAb) on adhesion of glioma cells to Fibronectin (FN) and the associated mechanisms. The effects of NRP-1 MAb on adhesion of U251 glioma cells and formation of stress fibers were studied using adhesion assays and confocal fluorescence microscopy. After treatment of U251 glioma cells with NRP-1 MAb, changes of related proteins adhering to FN were detected by co-immunoprecipitation and Western blot. Adhesion assays showed that NRP-1 MAb played an inhibitory role in adhesion of U251 glioma cells. Confocal fluorescence microscopy, co-immunoprecipitation and Western blot showed that NRP-1 MAb can inhibit the formation of stress fibers adhering to U251 cells. NRP-1 MAb can also affect the formation of Integrin alpha 5 beta 1-NRP-1 complex, and also reduce the level of phosphorylation of FAK and p130cas. In conclusion, NRP-1 MAb has the inhibitory effects on adhesion of glioma cells to FN. This effect is related to the inhibition of NRP-1 MAb on the formation of Integrin alpha 5 beta 1-NRP-1 complex, which leads to the inhibition of FAK/p130cas signaling pathway.