Ectopic expression of an apple apomixis-related gene MhFIE induces co-suppression and results in abnormal vegetative and reproductive development in tomato
Ectopic expression of an apple apomixis-related gene MhFIE induces co-suppression and results in abnormal vegetative and reproductive development in tomato
复制标题
苹果无融合生殖相关基因MhFIE的异位表达诱导共抑制并导致番茄营养和生殖发育异常
DOI：
10.1016/j.jplph.2012.07.018
复制
发表时间：
2012-12-01
影响因子：
4.3
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
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作者：
Liu, Dan-Dan;Dong, Qing-Long;Hao, Yu-Jin
在模式植物拟南芥中，不依赖受精的胚乳（FIE）在多种发育过程中起着重要的调控作用。然而，FIE基因在重要经济作物中的功能在很大程度上是未知的。本研究以无融合生殖海棠（Malus hupehensis Redh.）变种pingyiensis），将其导入番茄。半合子转基因番茄株系产生卷曲的叶片，种子发芽率降低。此外，共抑制的转基因MhFIE和内源基因（SlFIE）发生在纯合转基因番茄。结果表明，FIE基因沉默导致番茄生殖发育过程中出现异常表型，如花中萼片和花瓣数量增加，胚珠和雌蕊融合，单性结实果实形成等。酵母双杂交试验和双分子荧光互补（BiFC）证明MhFIE与番茄蛋白EZ 2（SlEZ 2）相互作用。其异位表达和SlFIE共抑制显著影响与叶、花和果实发育相关的基因的表达。因此，FIE与其他PcG蛋白一起，通过调控相关基因的表达，参与植物营养和生殖发育的调控。(C)2012 Elsevier GmbH. All rights reserved.
It has been well documented that FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) plays important regulatory roles in diverse developmental processes in model plant Arabidopsis thaliana. However, it is largely unknown how FIE genes function in economically important crops. In this study, MhFIE gene, which was previously isolated from apomictic tea crabapple (Malus hupehensis Redh. var. pingyiensis), was introduced into tomato. The hemizygous transgenic tomato lines produced curly leaves and decreased in seed germination. In addition, the co-suppression of the transgenic MhFIE and endogenous (SlFIE) genes occurred in homozygous transgenic tomatoes. As a result, FIE silencing brought about abnormal phenotypes during reproductive development in tomato, such as increased sepal and petal numbers in flower, a fused ovule and pistil and parthenocarpic fruit formation. A yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC) demonstrated that MhFIE interacted with a tomato protein, EZ2 (SlEZ2). Its ectopic expression and SlFIE co-suppression notably influenced the expression of genes associated with leaf, flower, and fruit development. Therefore, together with other PcG proteins, FIE was involved in the regulation of vegetative and reproductive development by modulating the expression of related genes in plants. (C) 2012 Elsevier GmbH. All rights reserved.