O2 and rat pulmonary artery tone: effects of endothelium, Ca2+, cyanide, and monocrotaline.
O2 and rat pulmonary artery tone: effects of endothelium, Ca2+, cyanide, and monocrotaline.
复制标题
O2 和大鼠肺动脉张力：内皮、Ca2、氰化物和野百合碱的影响。
DOI：
10.1152/jappl.1991.71.1.30
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Wolin,MS
中科院分区：
文献类型：
--
作者：
Mathew,R;Burke-Wolin,T;Gewitz,MH;Wolin,MS
本实验观察了内皮细胞（E）、Ca ~（2+）浓度、氰化物和野百合碱（MCT）预处理对大鼠肺门部肺动脉环（PA）缺氧反应的影响。在苯肾上腺素预收缩的PA中，缺氧诱导了初始E依赖性舒张相，随后是E非依赖性短暂收缩和最终舒张。Ca 2+浓度从1.5增加到2.5 mM产生了E-依赖性的减少音生成下O2和缺氧引起的初始松弛和短暂的收缩反应的显着增强。氰化物（0.1 mM）预收缩PA的添加产生了短暂的收缩缺氧引起的类似。预孵育氰化物导致抑制音的产生和取消的收缩缺氧。然而，最终的弛豫反应缺氧不抑制氰化物。因此，缺氧通过一种似乎也被氰化物激活的机制产生E-非依赖性收缩，并且该反应不被MCT改变。内皮细胞以Ca（2+）依赖的方式改变对缺氧的反应。
The present study examines the influence of the endothelium (E), Ca2+ concentration, cyanide and monocrotaline (MCT) pretreatment on the responses of isolated rat hilar pulmonary arterial rings (PA) to hypoxia. In PA precontracted with phenylephrine, hypoxia induced an initial E-dependent relaxation phase followed by an E-independent transient contraction and a final relaxation. An increase in Ca2+ concentration from 1.5 to 2.5 mM produced an E-dependent reduction in tone generation under O2 and a significant enhancement of the hypoxia-elicited initial relaxation and the transient contractile responses. Addition of cyanide (0.1 mM) to precontracted PA produced a transient contraction similar to that caused by hypoxia. Preincubation with cyanide led to inhibition of tone generation and abolition of the contraction to hypoxia. However, the final relaxation response to hypoxia was not inhibited by cyanide. Thus, hypoxia produces an E-independent contraction via a mechanism that appears also to be activated by cyanide, and this response is not altered by MCT. The endothelium alters the response to hypoxia in a Ca(2+)-dependent manner.