PEROXYNITRITE-MEDIATED OXIDATIVE PROTEIN MODIFICATIONS
PEROXYNITRITE-MEDIATED OXIDATIVE PROTEIN MODIFICATIONS
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DOI：
10.1016/0014-5793(95)00307-u
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发表时间：
1995-05-15
期刊：
影响因子：
3.5
通讯作者：
ALMEHDI, AB
中科院分区：
文献类型：
--
作者：
ISCHIROPOULOS, H;ALMEHDI, AB
蛋白质是活性物种的目标，氧化修饰蛋白质的检测通常被用作氧化应激的指标。过氧亚硝酸盐是一氧化氮与超氧化物反应形成的一种强氧化剂，以无脂肪酸的牛血清白蛋白为模型，研究了过氧亚硝酸盐介导的蛋白质修饰，过氧亚硝酸盐与蛋白质的反应导致色氨酸和半胱氨酸的氧化，酪氨酸的硝化反应，二叔丁氨酸的生成，2，4-二硝基苯肼反应性羰基的生成和蛋白质的断裂，3-硝基酪氨酸的形成代表了一种不同于活性氧物种的特殊过氧亚硝酸盐介导的蛋白质修饰。
Proteins are targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide, Using fatty acid-free bovine serum albumin as a model we examined peroxynitrite-mediated protein modifications, The reaction of protein with peroxynitrite resulted in the oxidation of tryptophan and cysteine, in the nitration of tyrosine, in the formation of dityrosine, in the production of 2,4 dinitrophenylhydrazine-reactive carbonyls and in protein fragmentation, The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species.