Aberrant phosphorylation of signal transducer and activator of transcription 3 protein of the CD4(+) T cells in patients with primary immune thrombocytopenia
Aberrant phosphorylation of signal transducer and activator of transcription 3 protein of the CD4(+) T cells in patients with primary immune thrombocytopenia
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原发性免疫性血小板减少症患者CD4( ) T细胞信号转导子和转录激活子3蛋白的异常磷酸化
DOI：
10.1097/mbc.0000000000000742
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发表时间：
2018
影响因子：
1.1
通讯作者：
Cheng Yunfeng
中科院分区：
文献类型：
--
作者：
Ke Yang;Li Feng;Zhan Yanxia;Wu Boting;Zou Shanhua;Ji Lili;Cheng Yunfeng;Min Zhihui;Hou Jiayun;Cheng Yunfeng;Li Feng;Sun Lihua;Cheng Yunfeng;Chen Hao;Cheng Yunfeng
原发性免疫性血小板减少症（ITP）是一种以血小板减少为特征的自身免疫性疾病，其发病机制是细胞免疫功能紊乱。信号转导子和转录激活子3（STAT 3）对T细胞的分化至关重要。本研究旨在探讨ITP患者CD 4 + T细胞STAT 3蛋白磷酸化的情况。其中14例初诊ITP患者经激素治疗后完全缓解（R组），15例初诊ITP患者经激素治疗后无反应（N组）。16名健康志愿者作为正常对照组（C组）。在治疗前后采集血样。ELISA法检测血清IL-6水平。流式细胞仪检测STAT 3蛋白磷酸化水平和Th 17细胞在CD 4 + T细胞中的比例。Real-time PCR检测STAT 3 mRNA的表达。R组IL-6水平高于C组（P= 0.02），而N组与C组间无差异。R组pSTAT 3基础水平显著高于N组（P= 0.002）。ITP患者外周血中Th 17细胞占CD 4 + T细胞的比例明显高于对照组（P= 0.03）。我们的研究结果表明，高水平pSTAT 3的ITP患者可能有更好的反应，皮质类固醇治疗，值得进一步研究pSTAT 3在ITP的预后作用。
Primary immune thrombocytopenia (ITP) is an autoimmune disorder characterized by decreased platelet count of which dysfunctional cellular immunity in its pathogenesis. Signal transducer and activator of transcription 3 (STAT3) is critical for the differentiation of T cells. The present study was aimed to investigate the STAT3 protein phosphorylation of CD4+ T cells in ITP patients. Fourteen patients of newly diagnosed ITP with complete remission (R group) and other 15 newly diagnosed ITP patients with nonresponse (N group) after corticosteroids therapy were included. Sixteen healthy human volunteers were served as normal controls (C group). Blood samples were collected before and after the therapy. Serum levels of IL-6 were measured by ELISA. The phosphorylation of STAT3 protein (pSTAT3) and the percentage of Th17 cells of the CD4+ T cells were analyzed by flow cytometry. The STAT3 mRNA expression was examined by Real-time PCR. The level of IL-6 in the R group was higher than that in the C group (P= 0.02) whereas no difference was found between groups of N and C. The basal level of pSTAT3 in the R group was significantly higher when compared with N group (P= 0.002). The percentage of Th17 cells of the CD4+ T cells in the ITP patients was numerically higher than that in the controls (P= 0.03). Our results indicate that ITP patient with higher basal level of pSTAT3 might have more favorite response to the corticosteroid therapy, which warrants further investigation on the prognostic role of pSTAT3 in ITP.