Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis
Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis
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DOI：
10.1128/iai.67.3.1045-1049.1999
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发表时间：
1999-03-01
影响因子：
3.1
通讯作者：
Tarkowski, N
中科院分区：
文献类型：
--
作者：
Nilsson, IM;Hartford, O;Tarkowski, N
脓毒性关节炎是葡萄球菌感染的常见和可怕的并发症。金黄色葡萄球菌产生许多潜在的毒力因子，包括某些粘附素和肠毒素。在这项研究中，我们已经评估了溶细胞毒素的作用，在脓毒性关节炎的发展与金黄色葡萄球菌野生型菌株8325-4或同基因突变体的表达不同的α，β和γ毒素生产模式的小鼠。接种α毒素或β毒素突变体的小鼠表现出与野生型接种小鼠相似的炎症、关节损伤和体重减轻程度。相比之下，与接种野生型菌株的小鼠相比，接种双重（α和γ毒素缺陷）或三重（α，β和γ毒素缺陷）突变金黄色葡萄球菌菌株的小鼠显示出较低的关节炎频率和严重程度，临床和组织学测量。我们的结论是，同时生产的α-和γ-毒素是一个毒力因子，金黄色葡萄球菌关节炎。
Septic arthritis is a common and feared complication of staphylococcal infections. Staphylococcus aureus produces a number of potential virulence factors including certain adhesins and enterotoxins. In this study we have assessed the roles of cytolytic toxins in the development of septic arthritis by inoculating mice with S, aureus wild-type strain 8325-4 or isogenic mutants differing in the expression of alpha-, beta-, and gamma-toxin production patterns. Mice inoculated with either an alpha- or beta-toxin mutant showed degrees of inflammation, joint damage, and weight decrease similar to wild-type-inoculated mice. In contrast, mice inoculated with either double (alpha- and gamma-toxin-deficient)- or triple (alpha-, beta-, and gamma-toxin-deficient)mutant S, aureus strains showed lower frequency and severity of arthritis, measured both clinically and histologically, than mice inoculated with the wild-type strain. We conclude that simultaneous production of alpha- and gamma-toxin is a virulence factor in S, aureus arthritis.