Early exclusive use of the affected forelimb after moderate transient focal ischemia in rats - Functional and anatomic outcome
Early exclusive use of the affected forelimb after moderate transient focal ischemia in rats - Functional and anatomic outcome
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DOI：
10.1161/01.str.31.5.1144
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发表时间：
2000-05-01
期刊：
影响因子：
8.3
通讯作者：
Grotta, JC
中科院分区：
文献类型：
--
作者：
Bland, ST;Schallert, T;Grotta, JC
背景和目的-我们实验室的研究人员之前的工作表明，在大鼠的早期关键期单独使用受影响的前肢会夸大病变体积，并延缓电解损伤前肢感觉运动皮质后的功能恢复。在本研究中，我们观察了大脑中动脉闭塞(MCAO)后单独使用受影响的前肢的效果。方法在雄性Long-Evans大鼠左侧大脑中动脉和双侧颈总动脉闭塞45min后，造成中度缺血。分别用石膏固定同侧(MCAO+IPSI)或对侧(MCAO+对侧)前肢10天，或不打石膏(MCAO+NOCAST)。进行假手术，动物也被铸型10天或不铸型。感觉运动测试在第17天至38天进行。在感觉运动测试结束时，使用Morris水迷宫测试认知能力。在另一项单独的实验中，在45分钟的缺血和铸型后的10天内测量温度和皮质酮水平。结果：MCAO+ipsi组在感觉运动任务中的表现比MCAO+对比组、MCAO+nocast组和假手术组差。MCAO+ipsi组心肌梗死体积明显大于Sham组和MCAO+对照组，而MCAO+nocast组无明显差异。在Morris水迷宫中没有发现组间差异，在温度和血浆皮质酮水平上也没有组间差异。结论：局部缺血后立即单独使用受影响的前肢对感觉运动功能有不利影响，这种影响不能归因于高温或应激。
Background and Purpose-Previous work by researchers in our laboratory has shown that in the rat, the exclusive use of the affected forelimb during an early critical period exaggerates lesion volume and retards functional recovery after electrolytic lesions of the forelimb sensorimotor cortex. In the present study, we examined the effects of exclusive use of the affected forelimb after middle cerebral artery occlusion (MCAO).Methods-Ischemia of moderate severity was produced in male Long-Evans rats through 45 minutes of occlusion of the left middle cerebral and both common carotid arteries. Exclusive use of either the affected or unaffected forelimb was forced through immobilization of either the ipsilateral (MCAO+ipsi) or contralateral (MCAO+contra) forelimb, respectively, for 10 days in a plaster cast, or the animal was left uncasted (MCAO+nocast). Sham surgeries were performed, and animals were also casted for 10 days or left uncasted. Sensorimotor testing was performed during days 17 to 38. At the end of sensorimotor testing, cognitive performance was tested with use of the Morris water maze. In a separate experiment, temperatures and corticosterone levels were measured during the 10-day period after 45-minute ischemia and casting.Results-The MCAO+ipsi group performed worse on sensorimotor tasks than the MCAO+contra, MCAO+nocast, and sham groups. Infarct volume was significantly larger in the MCAO+ipsi group than in the sham and MCAO+contra groups but not in the MCAO+nocast group. No group differences were found with the Morris water maze, and no group differences were found in either temperature or plasma corticosterone level.Conclusion-The exclusive use of the affected forelimb immediately after focal ischemia has detrimental effects on sensorimotor function that cannot be attributed to hyperthermia or stress.