Hippocampal neurogenesis of Wistar Kyoto rats is congenitally impaired and correlated with stress resistance
Hippocampal neurogenesis of Wistar Kyoto rats is congenitally impaired and correlated with stress resistance
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DOI：
10.1016/j.bbr.2017.04.046
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发表时间：
2017-06-30
影响因子：
2.7
通讯作者：
Date, Isao
中科院分区：
文献类型：
--
作者：
Kin, Kyohei;Yasuhara, Takao;Date, Isao
海马体被认为是抑郁症的重要区域。然而，海马神经发生与抑郁症之间的关系仍存在争议。 Wistar京都（WKY）大鼠经常被用作抑郁症模型。众所周知，WKY 大鼠表现出生理异常特征，这些特征类似于抑郁症患者的异常特征。然而，WKY大鼠的神经发生仍不清楚。在本研究中，我们首先评估了 WKY 大鼠的神经发生，并将其与 Wistar (WIS) 大鼠进行了比较。室下区 (SVZ) 中 5-溴-2'-脱氧尿苷 (BrdU) 和 BrdU/双皮质素 (Dcx) 阳性细胞数量未观察到应变效应。然而，WKY 大鼠海马齿状回 (DG) 中 BrdU 和 BrdU/Dcx 阳性细胞的数量显着低于 WIS 大鼠。接下来，我们评估了神经发生和行为测试之间的相关性。行为测试不影响这两种菌株的神经发生。每种品系的海马神经发生与第 2 天强迫游泳测试 (FST) 的结果呈负相关。也就是说，DG 中天然神经发生水平较低的大鼠在第一天表现出较高水平的习得性无助，这是由 FST 不可避免的压力引起的。我们的研究结果表明，与 WIS 大鼠相比，WKY 大鼠的海马神经发生先天受损。 DG 中的天然细胞增殖和神经发生与应激抵抗力相关。这些发现可能有助于制定抑郁症治疗的新目标。
The hippocampus is thought to be an important region for depression. However, the relationship between hippocampal neurogenesis and depression is still controversial. Wistar Kyoto (WKY) rats are frequently used as a depression model. WKY rats are known to show physiologically abnormal features, and these features resemble abnormalities seen in depressed patients. However, the neurogenesis of WKY rats is still unknown. In this study, we first evaluated the neurogenesis of WKY rats and compared it to that of Wistar (WIS) rats. No strain effect was observed in the number of cells positive for 5-bromo-2'-deoxyuridine (BrdU) and BrdU/Doublecortin (Dcx) in the subventricular zone (SVZ). However, the number of BrdU-and BrdU/Dcx-positive cells in the dentate gyrus (DG) of the hippocampus was significantly lower in WKY rats than in WIS rats. Next, we evaluated the correlation between neurogenesis and behavior tests. Behavior tests did not affect neurogenesis in either strain. Hippocampal neurogenesis correlated negatively with the results of a forced swim test (FST) on day 2 in each strain. That is, rats with a lower level of native neurogenesis in the DG showed a higher level of learned helplessness induced by the inescapable stress of the FST on day 1.Our findings indicate that hippocampal neurogenesis in WKY rats is congenitally impaired in contrast to that in WIS rats. Native cell proliferation and neurogenesis in the DG are correlated with stress resistance. These findings may be useful for developing new targets for depression treatment.