Ultrasensitive Faraday cage-type electrochemiluminescence assay for femtomolar miRNA-141 via graphene oxide and hybridization chain reaction-assisted cascade amplification
Ultrasensitive Faraday cage-type electrochemiluminescence assay for femtomolar miRNA-141 via graphene oxide and hybridization chain reaction-assisted cascade amplification
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通过氧化石墨烯和杂交链式反应辅助级联放大对飞摩尔 miRNA-141 进行超灵敏法拉第笼式电化学发光测定
DOI：
10.1016/j.bios.2018.02.062
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发表时间：
2018-06-30
影响因子：
12.6
通讯作者：
Guo, Zhiyong
中科院分区：
文献类型：
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作者：
Lu, Jing;Wu, Lin;Guo, Zhiyong
本研究在Faraday笼型策略的基础上，通过氧化石墨烯(GO)和杂交链反应(HCR)辅助的级联扩增，构建了一种新型的电致化学发光(ECL)生物传感器，用于敏感地检测毫微米RNA-141。将捕获探针(CP)固定在Fe3O4@SiO_2@Au纳米粒子上作为捕获单元，可以捕获miRNA-141，并通过核酸杂交实现了信号单元(Ru(Phen)(3)(2+)-hcr/go)的固定化。所制备的生物传感器在信号放大方面有两个优点：第一，GO可以直接搭接在电极表面，由于具有较大的表面积和良好的电子传输特性，延长了传感器的外亥姆霍兹平面(OHP)；第二，HCR辅助的级联放大是通过将所有HCR产物锚定在GO表面，然后嵌入Ru(Phen)(3)(2+)作为信号读出途径来设计的。所有这些信号分子都可以参与电化学反应，从而进一步极大地增强ECL信号。因此，将HCR和Faraday笼型策略相结合构建的传感器对miRNA-141具有超灵敏的检测平台，检测下限为0.03fM。此外，这种生物传感器为分析其他microRNAs提供了一个通用的平台。
In this study, a novel electrochemiluminescence (ECL) biosensor for sensitive detection of femtomolar miRNA-141 was constructed on the basis of Faraday cage-type strategy via graphene oxide (GO) and hybridization chain reaction (HCR)-assisted cascade amplification. A capture probe (CP) was immobilized on Fe3O4@SiO2@Au nanoparticles as capture unit, which could catch the miRNA-141, and the immobilization of the signal unit (Ru (phen)(3)(2+)-HCR/GO) was allowed via nucleic acid hybridization. The prepared biosensor exhibited two advantages for signal amplification: firstly, GO could lap on the electrode surface directly, extending Outer Helmholtz Plane (OHP) of the sensor due to the large surface area and good electronic transport property; secondly, HCR-assisted cascade amplification was designed by anchoring all HCR products on the GO surface, then embedding Ru(phen)(3)(2+) as a signal readout pathway. All these signal molecules could take part in electrochemical reactions, thus further enhancing the ECL signal drastically. Therefore, the proposed sensor constructed by integrating HCR with Faraday cage-type strategy displayed an ultrasensitive detection platform for the miRNA-141 with a low detection limit of 0.03 fM. In addition, this proposed biosensor provides a universal platform for analysis of other microRNAs.