Follistatin-like 1 as a Novel Adipomyokine Related to Insulin Resistance and Physical Activity
Follistatin-like 1 as a Novel Adipomyokine Related to Insulin Resistance and Physical Activity
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Follistatin-like 1 作为一种与胰岛素抵抗和体力活动相关的新型脂肪肌因子
DOI：
10.1210/clinem/dgaa629
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发表时间：
2020-12-01
影响因子：
5.8
通讯作者：
Yang, Gangyi
中科院分区：
文献类型：
--
作者：
Xu, Xiaohui;Zhang, Tingran;Yang, Gangyi
背景和目标：卵泡抑素样蛋白-1（FSTL-1）被认为是一种脂肪因子或肌因子，可能是一种潜在的代谢调节因子。方法：采用定量逆转录聚合酶链反应（qRT-PCR）和蛋白质印迹法检测C57 BL/6 J和db/db小鼠外周血FSTIA的表达，并分析其与2型糖尿病（T2 DM）胰岛素抵抗（IR）的关系。采用酶联免疫吸附法检测298例2型糖尿病患者和202例健康对照者血清FSTL-1水平。在口服葡萄糖耐量试验，EHC（正常血糖-高胰岛素钳夹），脂质输注，急性运动，冷暴露test.Results：我们发现，FSTL-1蛋白在db/db小鼠的脂肪组织中的表达显着高于野生型小鼠的循环FSTLA水平的变化。重要的是，T2 DM和超重/肥胖参与者的循环FSTL-1水平高于健康和瘦个体，并且与HOMA-IR、脂联素以及肥胖和代谢相关参数相关。在干预研究中，发现45分钟的体力活动可以显着增加年轻健康参与者的循环FSTL-1浓度。此外，小鼠脂肪组织中的FSTL-1蛋白表达响应于体力活动而显著升高。EHC期间的高胰岛素血症和脂质输注诱导的FFA急性升高导致循环FSTL 1水平显著降低。然而，没有变化被发现在循环中的FSTL-1水平响应于口服葡萄糖挑战或冷暴露test.Conclusions：FSTL-1可能是一种脂肪肌因子与胰岛素抵抗和体力活动，和循环FSTL-1水平增加T2 DM患者。
Background and Aims: Follistatin-like protein-1 (FSTL-1) is considered to be an adipokine or myokine that could be a potential regulator of metabolism. Our purpose is to investigate the relationship between circulating FSTL-1 levels and insulin resistance (IR) in type 2 diabetes mellitus (T2DM) and to identify the regulatory factors.Methods: FSTIA expression in C57BL/6J and db/db mice was examined by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and Western blots. Serum FSTL-1 levels were measured by enzyme-linked immunosorbent assay in 298 T2DM patients and 202 healthy controls. Changes in the circulating FSTLA level were observed during the oral glucose tolerance test, EHC (euglycemic-hyperinsulinemic clamp), lipid infusion, acute exercise, and cold-exposure test.Results: We found that FSTL-1 protein expression in the adipose tissue of db/db mice was significantly higher than that of wild-type mice. Importantly, circulating FSTL-1 levels in T2DM and overweight/obese participants were higher than those in healthy and lean individuals, and was related to HOMA-IR, adiponectin, and obesity- and metabolism-related parameters. In the intervention study, 45 minutes of physical activity was found to significantly increase the circulating FSTL-1 concentration in young, healthy participants. Further, FSTL-1 protein expression in adipose tissue rose dramatically in response to physical activity in mice. Hyperinsulinemia during EHC and acute elevated FFA induced by lipid infusion resulted in a significant decrease in the circulating FSTL1 levels. However, no change was found in the circulating FSTL-1 levels in response to the oral glucose challenge or cold-exposure test.Conclusions: FSTL-1 may be an adipomyokine associated with insulin resistance and physical activity, and circulating FSTL-1 levels are increased in patients with T2DM.