The identification of 3:5:3'-L-triiodothyronine in human plasma.
The identification of 3:5:3'-L-triiodothyronine in human plasma.
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人血浆中 3:5:3-L-三碘甲状腺原氨酸的鉴定。
DOI：
10.1016/s0140-6736(52)91952-1
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发表时间：
1952
期刊：
影响因子：
168.9
通讯作者：
R. Pitt
中科院分区：
文献类型：
--
作者：
J. Gross;R. Pitt
方法对所列患者在给予放射性碘后的不同时间间隔进行血浆取样。血浆酸化，用丁醇提取，丁醇提取物如前所述蒸发。应该注意的是，在蒸发过程中，样品中存在的大部分碘化物都丢失了（图2d）。将患者1和患者2的样品与100µg合成的三碘甲状腺原氨酸和甲状腺素一起放在纸上，用两对溶剂——丁醇-二恶氨对抗丁醇-乙酸（图1），丁醇-二恶氨对抗碰撞碱-氨——进行二维发育。后一种溶剂是根据Taurog等人（1950）制备的，它本身不能将三碘甲状腺原氨酸从甲状腺素中分离出来。
METHODSSamples of plasma were obtained from the series of patients shown in the accompanying table at various time-intervals after the administration of the radioactive iodine. The plasmas were acidified, and extracted with butanol, and the butanol extracts were evaporated as described previously. It should be noted that in the course of evaporation most of the iodide present in the sample is lost (fig. 2d). The samples from patients 1 and 2 were placed on paper, together with 100 µ g. of synthetic triiodothyronine and thyroxine, and developed two-dimensionally with two solvent pairs-butanol-dioxan-ammonia against butanol-acetic acid (fig. 1), and butanol-dioxan-ammonia against collidine-ammonia. The latter solvent was pre-pared according to Taurog et al.(1950), and by itself did not separate triiodothyronine from thyroxine.