Motion-gated acquisition for in vivo optical imaging
Motion-gated acquisition for in vivo optical imaging
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DOI：
10.1117/1.3275473
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发表时间：
2009-11-01
影响因子：
3.5
通讯作者：
Frangioni, John V.
中科院分区：
文献类型：
--
作者：
Gioux, Sylvain;Ashitate, Yoshitomo;Frangioni, John V.
宽场连续波荧光成像、荧光寿命成像、频域光子迁移和空间调制成像具有提供体内定量测量的潜力。然而，由于具有挑战性的环境限制，这些技术中的大多数尚未成功地转化为临床。在许多情况下，心脏和呼吸运动极大地损害图像质量和/或定量处理。为了解决这一根本问题，我们已经开发出一种低成本，现场可编程门阵列为基础的，只有硬件的门控设备，提供了一个锁相采集窗口的任意延迟和宽度，是从一个无限数量的伪周期性和非周期性的输入信号。所有设备功能都可以手动或通过USB串行命令进行控制。该设备的工作范围涵盖了小鼠心电图（每分钟1000次）到人类呼吸（每分钟4次呼吸）的极端情况，具有定时分辨率
Wide-field continuous wave fluorescence imaging, fluorescence lifetime imaging, frequency domain photon migration, and spatially modulated imaging have the potential to provide quantitative measurements in vivo. However, most of these techniques have not yet been successfully translated to the clinic due to challenging environmental constraints. In many circumstances, cardiac and respiratory motion greatly impair image quality and/or quantitative processing. To address this fundamental problem, we have developed a low-cost, field-programmable gate array-based, hardware-only gating device that delivers a phase-locked acquisition window of arbitrary delay and width that is derived from an unlimited number of pseudo-periodic and nonperiodic input signals. All device features can be controlled manually or via USB serial commands. The working range of the device spans the extremes of mouse electrocardiogram (1000 beats per minute) to human respiration (4 breaths per minute), with timing resolution