Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells
Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells
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DOI：
10.1042/cbi20090248
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发表时间：
2010-06-01
影响因子：
3.9
通讯作者：
Hui, Guozhen
中科院分区：
文献类型：
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作者：
Dong, Wanli;Chen, Haiyun;Hui, Guozhen
本研究探讨了人羊膜上皮细胞（human amniotic epithelial cells，hAECs）移植到脑出血大鼠侧脑室后对脑水肿和神经功能恢复的影响。从人足月胎盘中分离hAEC并接种用于原代培养。我们将Hoechst 33258标记的hAECs和用慢病毒载体转染的增强型绿色荧光蛋白（EGFP）基因导入脑出血大鼠模型。28天后评价动物的行为和脑水肿，并制作脑切片，用荧光显微镜进行形态学和免疫组织化学分析。我们的结果如下。移植的hAECs可沿着外侧壁生长，并存活至少4周。部分细胞用抗波形蛋白和巢蛋白的人特异性抗体染色。在损伤部位周围，用OX42染色的活化的小胶质细胞减少。治疗组脑出血大鼠脑含水量降低。治疗组的行为测试评分较对照组有所改善。结论：hAECs移植后能在脑出血大鼠侧脑室存活，并表达vimentin和nestin。hAEC移植可减轻脑出血大鼠的脑水肿，改善脑出血大鼠的运动障碍。
We explored the effects on brain oedema and neurological functional recovery after transplantation of hAECs (human amniotic epithelial cells) into the lateral ventricle of rats with ICH (intracerebral haemorrhage). hAECs were isolated from human term placenta and seeded for primary culture. We delivered hAECs labelled with Hoechst33258 and transfected with EGFP (enhanced green fluorescent protein) gene using lentiviral vectors into ICH rat models. The behaviour of the animals and brain oedema were evaluated after 28 days, and brain sections were made for morphological and immunohistochemical analyses with fluorescence microscopy. Our results were as follows. Transplanted hAECs were observed along the lateral wall and survived for at least 4 weeks. Some of the cells were stained with human specific antibody to vimentin and nestin. Around the injury site, activated microglia stained with OX42 were reduced. The water content of ICH rats decreased in the treatment group. The behaviour test scores were improved in the treatment group compared with those in the control groups. In conclusion, hAECs cannot only survive in the lateral ventricle of ICH rats after transplantation, but also express vimentin and nestin. hAEC transplantation reduced brain oedema and improved the motor deficits of ICH rats.