Measuring Nanoscale Forces with Living Probes
Measuring Nanoscale Forces with Living Probes
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DOI：
10.1021/nl303585w
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发表时间：
2012-11-01
期刊：
影响因子：
10.8
通讯作者：
Carberry, D. M.
中科院分区：
文献类型：
--
作者：
Olof, S. N.;Grieve, J. A.;Carberry, D. M.
光捕获技术已被用于研究基本的生物过程，从识别驱动蛋白和肌球蛋白的加工机制到了解 DNA 的机制。迄今为止，这些研究几乎完全依赖于基于胶体微球的各向同性探针的使用。然而，利用更复杂的探针形态有许多潜在的优势：使用多个捕获点可以控制相互作用体积；增加光阱和样品之间的距离可最大限度地减少敏感生物材料的光损伤；几何各向异性引入了不对称表面化学和多功能探针的潜力。在这里，我们证明淡水硅藻 Nitzschia subacillaryis Hustedt 的活细胞可以用作全息光学镊子应用的先进探针。我们表征了与硅藻壳的高形状各向异性相关的光学和材料特性，检查了活藻细胞的捕获行为，并演示了如何校准硅藻以在大鼠 B 细胞杂交瘤 (11B11) 细胞存在的情况下用作力传感器和力探针。
Optical trapping techniques have been used to investigate fundamental biological processes ranging from the identification of the processive mechanisms of kinesin and myosin to understanding the mechanics of DNA. To date, these investigations have relied almost exclusively on the use of isotropic probes based on colloidal microspheres. However, there are many potential advantages in utilizing more complex probe morphologies: use of multiple trapping points enables control of the interaction volume; increasing the distance between the optical trap and the sample minimizes photodamage in sensitive biological materials; and geometric anisotropy introduces the potential for asymmetric surface chemistry and multifunctional probes. Here we demonstrate that living cells of the freshwater diatom Nitzschia subacicularis Hustedt can be exploited as advanced probes for holographic optical tweezing applications. We characterize the optical and material properties associated with the high shape anisotropy of the silica frustule, examine the trapping behavior of the living algal cells, and demonstrate how the diatoms can be calibrated for use as force sensors and as force probes in the presence of rat B-cell hybridoma (11B11) cells.