Genomic basis of white pine blister rust quantitative disease resistance and its relationship with qualitative resistance
Genomic basis of white pine blister rust quantitative disease resistance and its relationship with qualitative resistance
复制标题
白松锈病定量抗性的基因组基础及其与定性抗性的关系
DOI：
10.1111/tpj.14928
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
De La Torre, Amanda R.
中科院分区：
文献类型：
--
作者：
Weiss, Matthew;Sniezko, Richard A.;Puiu, Daniela;Crepeau, Marc W.;Stevens, Kristian;Salzberg, Steven L.;Langley, Charles H.;Neale, David B.;De La Torre, Amanda R.
控制数量抗病基因座变异的基因组结构和分子机制在植物物种中还没有得到很好的理解，并且在长世代树中几乎没有研究。将数量性状基因座定位和全基因组关联研究相结合，以测试一个大型单核苷酸多态性（SNP）集与糖松中定量和定性白松疱锈病抗性的关联。在没有染色体规模参考基因组的情况下，生成高密度共有连锁图谱以获得相关SNP的位置。新发现的与白松疱锈病定量抗病性相关的453个SNP涉及广泛的生物学功能，包括与抗病性相关的基因和其他参与形态和发育过程的基因。此外，NBS‐LRR病原识别基因被发现参与数量抗病性，这表明这些新报道的基因是具有部分抗性的质量基因，它们是由于无毒小种导致质量抗性失败的结果，或者它们对质量抗病基因具有上位性作用。这项研究是我们理解长世代树木数量抗病性的复杂基因组结构的一步，也是白松物种标记辅助抗病育种的第一步。
The genomic architecture and molecular mechanisms controlling variation in quantitative disease resistance loci are not well understood in plant species and have been barely studied in long‐generation trees. Quantitative trait loci mapping and genome‐wide association studies were combined to test a large single nucleotide polymorphism (SNP) set for association with quantitative and qualitative white pine blister rust resistance in sugar pine. In the absence of a chromosome‐scale reference genome, a high‐density consensus linkage map was generated to obtain locations for associated SNPs. Newly discovered associations for white pine blister rust quantitative disease resistance included 453 SNPs involved in wide biological functions, including genes associated with disease resistance and others involved in morphological and developmental processes. In addition, NBS‐LRR pathogen recognition genes were found to be involved in quantitative disease resistance, suggesting these newly reported genes are qualitative genes with partial resistance, they are the result of defeated qualitative resistance due to avirulent races, or they have epistatic effects on qualitative disease resistance genes. This study is a step forward in our understanding of the complex genomic architecture of quantitative disease resistance in long‐generation trees, and constitutes the first step towards marker‐assisted disease resistance breeding in white pine species.