Cell proliferation and invasion in malignant gliomas.
Cell proliferation and invasion in malignant gliomas.
复制标题
恶性胶质瘤中的细胞增殖和侵袭。
作者：
D. Schiffer;P. Cavalla;A. Dutto;L. Borsotti
在60例恶性胶质瘤活检中研究了细胞增殖和侵袭，以重现90例胶质母细胞瘤尸检病例中确定的扩散方式。用PCNA和MIB-1的免疫组化染色及计算其标记指数（LI）来观察细胞增殖情况。主要发现是细胞增殖和细胞侵袭不一定相关。实体瘤和邻近脑之间的界面由肿瘤细胞密度梯度或肿瘤的明确分界表示。在第一种情况下，LI在浸润区域与实体瘤相比没有变化，或者低得多，而在第二种情况下，在肿瘤周边有一个高密度标记核的环。神经元周卫星细胞增殖标记常呈阳性。来自深部肿瘤的外皮层细胞几乎为阴性，而来自蛛网膜下或软膜下浸润的细胞显示高LI。在蛛网膜下和软膜下生长，以及在脑膜穿透血管周围下降到脑实质的细胞群中也发现了高LI。从体内研究来看，细胞增殖和侵袭之间的关系并不是直接和简单的。
Cell proliferation and invasion were studied in sixty biopsies of malignant gliomas selected to reproduce the spreading modalities identified in ninety autopsy cases of glioblastoma. Cell proliferation was studied by the immunohistochemical demonstration of PCNA and MIB-1 and by the calculation of their labeling indexes (LI). The main finding was that cell proliferation and cell invasion are not necessarily associated. The interface between the solid tumor and the adjacent brain was represented either by a gradient of tumor cell density or by a clearcut demarcation of the tumor. In the first case the LI either did not change in the infiltration area in comparison with solid tumor or it was much lower, whereas in the second case there was a ring with a high density of labeled nuclei at the tumor periphery. Perineuronal satellites were usually positive for proliferation markers. Cells accumulated in the outer cortical layers, from a deeply located tumor, were almost negative, whereas those originating from subarachnoidal or subpial invasion showed a high LI. High LIs were also found in subarachnoidal and subpial growths, and in a cell population descending into the brain parenchyma around meningeal penetrating vessels. The relationship between cell proliferation and invasion from in vivo studies is not a direct and a simple one.