COMMITMENT OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM TO SEXUAL AND ASEXUAL DEVELOPMENT
COMMITMENT OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM TO SEXUAL AND ASEXUAL DEVELOPMENT
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DOI：
10.1017/s0031182000061199
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发表时间：
1990-04-01
期刊：
影响因子：
2.4
通讯作者：
CARTER, R
中科院分区：
文献类型：
--
作者：
BRUCE, MC;ALANO, P;CARTER, R
血期疟原虫在脊椎动物宿主体内既可以发育成无性繁殖的分裂体，也可以发育成配子体，即寄生虫的有性阶段。本研究利用恶性疟原虫有性或无性期特异性单克隆抗体，研究了恶性疟原虫3D7A单克隆分裂体的子代向无性寄生虫或配子体的分化。我们观察到，从连续培养中获得的分裂体，经过一系列的生长周期和新鲜红细胞的稀释，要么只产生配子体，要么只产生无性寄生虫，显示出高度的一种或另一种发育途径。在低寄生密度条件下，分裂体产生配子体的相对比例非常低，而在高寄生密度条件下，分裂体产生配子体的比例要高得多。然而，在低和高寄生虫密度下，分裂体几乎总是完全致力于只产生配子体或只产生无性寄生虫。
Blood-stage malaria parasites in the vertebrate host can develop either into the asexual, multiplying forms, called schizonts, or into gametocytes, the sexual stages of the parasite. In the present work we studied the differentiation into asexual parasites or gametocytes of the progeny of single, isolated schizonts of the clone 3D7A of Plasmodium falciparum, using monoclonal antibodies specific for the sexual or asexual stages of the parasite. We observed that schizonts obtained from a continuous culture undergoing serial cycles of growth and dilution with fresh red blood cells produced either only gametocytes or only asexual parasites, showing a high degree of commitment to one or the other developmental pathway. The relative proportion of schizonts which produced gametocytes was very low at low parasite densities in culture, while at high parasite densities a much greater proportion of schizonts produced gametocytes. Nevertheless, at both low and high parasite densities individual schizonts were almost always fully committed to producing only gametocytes or only asexual parasites.