1H magnetic resonance spectroscopic imaging of permanent focal cerebral ischemia in rat:: longitudinal metabolic changes in ischemic core and rim
1H magnetic resonance spectroscopic imaging of permanent focal cerebral ischemia in rat:: longitudinal metabolic changes in ischemic core and rim
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DOI：
10.1016/s0006-8993(01)02579-3
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发表时间：
2001-07-13
期刊：
影响因子：
2.9
通讯作者：
Terashi, A
中科院分区：
文献类型：
--
作者：
Igarashi, H;Kwee, IL;Terashi, A
本研究的目的是利用H-1磁共振波谱成像（MRSI）确定永久性局灶性脑缺血中代谢物的区域差异是否可以按时间顺序观察，以及这些变化是否反映了病理结果。用H-1磁共振成像（H-1 MRSI）对大鼠永久性局灶性缺血后代谢物的区域变化进行了为期7天的纵向研究，并与组织病理学结果进行了比较。缺血诱导后4小时。n -乙酰- l-天冬氨酸（NAA）水平在外侧尾壳核和体感觉皮层的核心缺血区分别下降了22%和40%。NAA的减少伴随着乳酸的显著增加。然而，在内侧尾壳核缺血边缘，尽管乳酸显著增加，NAA仍被保留。缺血24 h后，尾壳核内侧（尾状核缺血边缘）NAA水平也显著降低。而扣带皮层（皮质缺血边缘）的NAA在24 ~ 48 h间下降更为缓慢，这种区域差异反映了急性期代谢紊乱的严重程度。缺血96 h后，h -1 MRSI检测到的所有代谢物水平均下降，NAA水平下降反映了组织病理损伤的严重程度。总之，H-1磁共振成像可以按时间顺序评估区域代谢差异。NAA下降的深度反映了慢性期的组织病理变化。(C) 2001 Elsevier Science B.V.版权所有
The purpose of this study was to determine whether regional differences in metabolites can be seen chronologically in permanent focal cerebral ischemia using H-1 magnetic resonance spectroscopic imaging (MRSI), and whether these changes reflect pathological outcome. Regional variation in metabolites after permanent focal ischemia were, investigated longitudinally in rats using H-1 MRSI for a total of 7 days and then compared to histopathological findings. Four hours after the induction of ischemia. N-acetyl-L-aspartate (NAA) levels in the lateral caudo-putamen and the somatosensory cortex, core ischemic regions, decreased 22 and 40%, respectively. This reduction in NAA was coupled with a marked rise in lactate. In the medial caudo-putamen, the ischemic rim, however, NAA was preserved in spite of a marked increase in lactate. By 24 h post ischemia, the levels of NAA in medial caudo-putamen (ischemic rim in caudate) also decreased significantly. However NAA in cingulated cortex (ischemic rim in cortex) decreased more gradually between 24 and 48 h. This regional difference can reflect the severity of metabolic derangement in the acute stage. After 96 h following ischemia, the levels of all metabolites detected by H-1 MRSI had decreased and the levels of NAA decline reflected the severity of histopathological damage. In conclusion, the regional metabolic differences could be assessed by H-1 MRSI chronologically. and the depth of NAA decline reflected histopathological changes in the chronic stage. (C) 2001 Elsevier Science B.V. All rights reserved.