Transplanted Endothelial Progenitor Cells Augment the Survival Areas of Rat Dorsal Flaps
Transplanted Endothelial Progenitor Cells Augment the Survival Areas of Rat Dorsal Flaps
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DOI：
10.3727/000000003108747127
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发表时间：
2003-09
影响因子：
3.3
通讯作者：
Y. Kubota;K. Kishi;H. Satoh;Takara Tanaka;H. Nakajima;T. Nakajima
中科院分区：
文献类型：
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作者：
Y. Kubota;K. Kishi;H. Satoh;Takara Tanaka;H. Nakajima;T. Nakajima
内皮祖细胞（EPCs）已被确定在外周血中，并已被报道纳入缺血区域，如缺血后肢。在这项研究中，我们研究了EPCs移植是否有助于挽救体内手术皮瓣。同时定量比较移植EPCs与成熟内皮细胞（ECs）的新生血管能力。本研究采用体外培养的大鼠主动脉内皮细胞，经多次传代培养。从大鼠心脏的血液中获得EPCs。采用密度梯度离心法分离血样。收集轻密度单核细胞（MNCs），并在用大鼠血浆玻连蛋白包被的塑料板上培养。在培养第7天贴壁的细胞被认为是EPC。然后将PBS（对照）、EC或EPC（3.0 × 105悬浮在1.0 ml PBS中）注射到皮瓣的中间。术后7天观察皮瓣成活情况。EPC移植组与其他两组相比，生存期显著延长（p < 0.003）。EPC移植组与其他两组相比具有显著更多的血管造影可检测的血管（p < 0.003）和显著更高的毛细血管密度（p < 0.03）。共聚焦显微镜显示，EPCs被纳入增强的新生血管形成。这些结果表明，内皮祖细胞移植可能是有益的挽救外科皮瓣，和内皮细胞的新生血管能力是上级。
Endothelial progenitor cells (EPCs) have been identified in peripheral blood, and have been reported to be incorporated into ischemic regions such as the ischemic hindlimb. In this study, we examined whether or not transplantation of EPCs is useful for salvaging surgical flaps in vivo. At the same time, we quantitatively compared the neovascularization ability of transplanted EPCs and that of mature endothelial cells (ECs). ECs obtained from the aorta of rats by explantation and passaged several times were used in the present study. EPCs were obtained from the blood of rat hearts. The blood samples were separated by density gradient centrifugation. Light-density mononuclear cells (MNCs) were collected and cultured on plastic plates coated with rat plasma vitronectin. Cells attached at day 7 of culture were deemed to be EPCs. Then PBS (control), ECs, or EPCs (3.0 × 105 suspended in 1.0 ml PBS) were injected at the middle of a flap. Seven days after surgery, the survival lengths of the flaps were evaluated. EPC-transplanted groups revealed statistically significant augmentation of survival length compared with the other two groups (p < 0.003). EPC-transplanted groups had significantly more angiographically detectable blood vessels (p < 0.003) and significantly higher capillary density (p < 0.03) than the other two groups. Confocal microscopy revealed that EPCs were incorporated into enhanced neovascularization. These results suggest that transplantation of EPCs may be useful for salvaging surgical flaps, and EPCs are superior to ECs in neovascularization ability.