The regular supplementation with an antioxidant mixture decreases oxidative stress in healthy humans. Gender effect
The regular supplementation with an antioxidant mixture decreases oxidative stress in healthy humans. Gender effect
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DOI：
10.1016/j.cccn.2004.06.009
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发表时间：
2004-11-01
影响因子：
5
通讯作者：
Fraga, CG
中科院分区：
文献类型：
--
作者：
Actis-Goretta, L;Carrasqueda, F;Fraga, CG
背景：抗氧化剂的消耗被认为与增强抗氧化防御和预防自由基相关疾病有关。我们评估了定期补充抗氧化剂混合物是否会改变健康人的氧化应激参数。方法：采用高效液相色谱法测定血浆中丙二醛（MDA）、维生素E （α -生育酚）、β -胡萝卜素、泛醇-10；用荧光法测定血浆2-硫代巴比妥酸活性物质（TBARS）。这种补充剂含有106国际单位的维生素E、10毫克的β -胡萝卜素、60毫克的辅酶Q-10和40毫克的硒。结果：在10天的洗脱期后，16名健康、自由生活的成年人（31-48岁，9名女性，7名男性）在30天内每天服用补充剂。在第20天，血浆中维生素E （α -生育酚）、β -胡萝卜素和泛醇-10的浓度分别比基线值增加了45%、66%和104%。血浆MDA浓度在第20天（25%）和第30天（32%）降低。治疗期间血浆TBARS浓度稳定下降，在第30天与基线相比下降11%。按性别区分，男性在基线时MDA和TBARS血浆浓度均高于女性。然而，这种差异在添加30天后消失。结论：观察到的与氧化应激相关的变量的变化表明，脂溶性化合物的混合物具有有效的抗氧化作用，性别差异表明，男性应该比女性更早地优化抗氧化剂防御。(C) 2004 Elsevier B.V.版权所有
Background: Antioxidant consumption is claimed to be associated with improved antioxidant defenses and with the prevention of free radical-associated diseases. We evaluated if the regular supplementation with an antioxidant mixture modified oxidative stress parameters in healthy humans.Methods: Malondialdehyde (MDA), vitamin E (alpha-tocopherol), beta-carotene, and ubiquinol-10 were determined in plasma by HPLC; plasma 2-thiobarbituric acid reactive substances (TBARS) were evaluated fluorometrically. The supplement contained 106 IU vitamin E, 10 mg beta-carotene, 60 mg coenzyme Q-10, and 40 mug selenium.Results: After a 10-day wash out period, 16 healthy, free-living adults (31-48 years old; 9 women, 7 men) consumed the supplement daily during 30 days. At day 20, plasma concentration of vitamin E (alpha-tocopherol), beta-carotene, and ubiquinol-10 increased 45%, 66%, and 104%, respectively, over baseline values. Plasma MDA concentrations were reduced at days 20 (25%) and 30 (32%). Plasma TBARS concentrations decreased steadily during the treatment, reaching at day 30 an 11% decrease compared to baseline. Segregating by gender, both MDA and TBARS plasma concentrations were higher in men than in women at baseline. However, such differences disappeared after 30 days of supplementation.Conclusion: The observed modifications in variables associated with oxidative stress are indicative of an effective antioxidant action of the used mixture of lipid soluble compounds, and gender differences suggest that men should optimize their antioxidants defenses earlier in life than women. (C) 2004 Elsevier B.V. All rights reserved.