Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells:: involvement of 5-hydroxytryptamine2A receptors
Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells:: involvement of 5-hydroxytryptamine2A receptors
复制标题
DOI：
10.1093/intimm/dxg027
复制
发表时间：
2003-02-01
影响因子：
4.4
通讯作者：
Cavaillon, JM
中科院分区：
文献类型：
--
作者：
Cloëz-Tayarani, I;Petit-Bertron, AF;Cavaillon, JM
为了进一步了解血清素[5-羟色胺（5-HT）]在炎症中的体内意义，我们研究了其对脂多糖（LPS）刺激的人外周血单核细胞（PBMC）中肿瘤坏死因子（TNF）-α、IL-1 α、IL-β、IL-6、IL-10和IL-1受体拮抗剂产生的影响。5-HT抑制PBMC中TNF-α的产生并增加IL-1 β的产生。IL-1 β转化酶/caspase-1水平保持不变，表明5-HT的作用与IL-1 β成熟过程没有直接关系。TNF-α mRNA和IL-1 β mRNA的含量在5-HT存在下没有变化。5-HT对所研究的其他细胞因子的产生没有任何影响。5-HT对TNF-α产生的抑制作用被选择性5-HT 2A拮抗剂酮色林拮抗，并被选择性5-HT 2A/2C激动剂DOI模拟。这些发现表明，5-HT对TNF-α产生的抑制涉及PBMC中5-HT 2A受体亚型的参与。因此，我们通过Western印迹分析检测PBMC中5-HT 2A受体的存在。我们的数据支持5-HT通过其对PBMC中细胞因子产生的影响在炎症中的作用。
In order to provide additional insight into the in vivo significance of serotonin [5-hydroxytryptamine (5-HT)] in inflammation, we examined its effect on the production of tumor necrosis factor (TNF)-alpha, IL-1alpha, IL-beta, IL-6, IL-10 and IL-1 receptor antagonist in lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC). 5-HT inhibited TNF-alpha production and increased IL-1beta production in PBMC. The level of IL-1beta-converting enzyme/caspase-1 remained unchanged, suggesting that the effect of 5-HT is not directly related to the IL-1beta maturation process. TNF-alpha mRNA and IL-1beta mRNA content did not change in the presence of 5-HT. 5-HT did not have any effect on the production of other cytokines studied. The inhibitory effect of 5-HT on TNF-alpha production was antagonized by ketanserin, a selective 5-HT2A antagonist, and mimicked by DOI, a selective 5-HT2A/2C agonist. These findings suggest that the inhibition of TNF-alpha production by 5-HT involves the participation of the 5-HT2A receptor subtypes in PBMC. Accordingly, we detected the presence of 5-HT2A receptors in PBMC by Western blot analysis. Our data support a role of 5-HT in inflammation through its effect on cytokine production in PBMC.