Increased expression of ferritin in cerebral cortex after human traumatic brain injury
Increased expression of ferritin in cerebral cortex after human traumatic brain injury
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人脑外伤后大脑皮层铁蛋白表达增加
DOI：
10.1007/s10072-012-1214-7
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发表时间：
2013-07-01
影响因子：
3.3
通讯作者：
Hang, Chun-Hua
中科院分区：
文献类型：
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作者：
Liu, Huan-Dong;Li, Wei;Hang, Chun-Hua
尽管在过去的几年里有许多研究和改进，但创伤后继发性脑损伤的确切机制仍然不清楚。铁对几乎所有类型的细胞都是必不可少的，包括神经细胞。然而，在动物模型中，过量的铁已被证明有助于创伤后的脑损伤。铁蛋白作为脑内重要的铁处理蛋白，可能参与铁诱导的各种脑疾病的病理生理过程。因此，本研究的目的是研究铁蛋白在人挫伤脑组织中的表达。19例脑挫裂伤患者于伤后3 h ~ 17 d取19例脑挫裂伤标本，另取3例岩斜区脑膜瘤患者的正常颞极标本作为对照。分别采用实时定量PCR、免疫印迹和免疫组化方法检测铁蛋白H链的表达。取Perl反应进行铁染色。结果表明，人颅脑创伤后可上调脑挫伤周围皮质铁蛋白H链的表达。早期组（伤后≤12 h）铁蛋白明显升高，并持续至伤后48 h。铁蛋白-H-链的定位主要见于神经元样细胞，很少见于胶质样细胞。Perl反应显示铁阳性细胞多为胶质样细胞。提示铁和铁蛋白可能参与了TBI继发性脑损伤的发生，可作为TBI治疗的靶点。
Despite numerous researches and improvements in the past few years, the precise mechanisms underlying secondary brain injury after trauma remain obscure. Iron is essential for almost all types of cells, including nerve cells. However, excess of iron has been proved to contribute to the brain injury following trauma in animal models. As a key iron-handling protein in the brain, ferritin might be involved in iron-induced pathophysiological process of various brain disorders. Therefore, the current study was aimed to investigate the expression of ferritin in the human contused brain. Nineteen contused brain samples were obtained from 19 patients undergoing surgery for brain contusions 3 h–17 d after trauma, and three normal temporal pole samples from 3 patients with petroclival meningioma were collected as controls. Expression of ferritin-H-chain was measured by quantitative real-time polymerase chain reaction (PCR), western blot and immunohistochemistry, respectively. Perl’s reaction was taken for iron staining. The results showed that human traumatic brain injury (TBI) could up-regulate ferritin-H-chain in pericontusional cortex. A marked increase of ferritin was detected in the early group (≤12 h), and remained elevated for a long time till after 48 h post-injury. The location of ferritin-H-chain was found mainly at the neuron-like cells and seldom at glia-like cells. Perl’s reaction showed that most of the iron-positive cells were glia-like cells. These findings suggested that iron and ferritin might be involved in the secondary brain injury and could be therapeutic targets for patients with TBI.