Combination of chlorogenic acid and salvianolic acid B protects against polychlorinated biphenyls-induced oxidative stress through Nrf2
Combination of chlorogenic acid and salvianolic acid B protects against polychlorinated biphenyls-induced oxidative stress through Nrf2
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绿原酸和丹酚酸 B 的组合可通过 Nrf2 防止多氯联苯诱导的氧化应激。
DOI：
10.1016/j.etap.2016.08.004
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发表时间：
2016-09-01
影响因子：
4.3
通讯作者：
Li, Lei
中科院分区：
文献类型：
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作者：
Chen, Lijun;Li, Yuan;Li, Lei
咖啡酸衍生物（CADs）是一类具有多种生理和药理活性的植物化学物质。本研究旨在探讨CADs对PCB 126诱导的小鼠肝损伤和氧化应激的联合保护作用。本研究采用化学发光法，筛选出绿原酸（CGA）、丹参素B（Sal B）作为抗氧化剂。然后，小鼠灌胃给予60 mg/kg/d的CGA、Sal B和CGA + Sal B（1：1）3周，然后暴露于0.05 mg/kg/d的PCB 126 2周。结果表明，CGA、Sal B、CGA + Sal B预处理均能有效减轻PCB 126所致的肝损伤和细胞毒作用，提高超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、血红素加氧酶-1（HO-1）和核因子E2相关因子2（Nrf 2）的表达，其中以CGA + Sal B的效果最好，具有协同保护作用。综上所述，由于Nrf 2通过上调抗氧化基因的表达来调节细胞保护反应，我们认为CGA和Sal B对PCB 126诱导的组织损伤具有联合保护作用，Nrf 2信号通路可能参与其中。(C)© 2016 Elsevier B. V.版权所有。
Caffeic acid derivatives (CADs) are well-known phytochemicals with multiple physiological and pharmacological activities. This study aimed to investigate the combined protective effects of CADs on PCB126-induced liver damages and oxidative stress in mice. Here, we used chemiluminescence and chose chlorogenic acid (CGA), salvianolic acid B (Sal B) as the best antioxidants. Then, mice were intra-gastrically administered with 60 mg/kg/d CGA, Sal B, and CGA plus Sal B (1:1) for 3 weeks before exposing to 0.05 mg/kg/d PCB126 for 2 weeks. We found that pretreatment with CGA, Sal B, and CGA plus Sal B effectively attenuated liver injury and cytotoxicity caused by PCB126, but improved the expressions of superoxide dismutase (SOD), glutathione reduced (GSH), heme oxygenase-1 (HO-1) and nuclear factor E2-related factor 2 (Nrf2), CGA plus Sal B especially, was found to have the best effects that indicated a synergetic protective effect. Taken together, as the Nrf2 regulates the cyto-protective response by up-regulating the expression of antioxidant genes, we suggested that CGA plus Sal B had a combined protection on PCB126-induced tissue damages and that the Nrf2 signaling might be involved. (C) 2016 Elsevier B.V. All rights reserved.